PUBLICATION
Neurotrophin-6 is a new member of the nerve growth factor family
- Authors
- Götz, R., Köster, R.W., Winkler, C., Raulf, F., Lottspeich, F., Schartl, M., and Thoenen, H.
- ID
- ZDB-PUB-060314-9
- Date
- 1994
- Source
- Nature 372(6503): 266-269 (Journal)
- Registered Authors
- Köster, Reinhard W., Schartl, Manfred, Winkler, Christoph
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cell Division
- Cell Line
- Chick Embryo
- Cloning, Molecular
- Cyprinodontiformes/embryology
- Cyprinodontiformes/genetics
- Extracellular Matrix/metabolism
- Heparin/metabolism
- Humans
- Mice
- Molecular Sequence Data
- Nerve Growth Factors/classification
- Nerve Growth Factors/genetics*
- Nerve Growth Factors/metabolism
- Neurons/cytology
- Protein Binding
- Proteoglycans/metabolism
- Rabbits
- Recombinant Proteins
- Sequence Homology, Amino Acid
- Solubility
- PubMed
- 7969471 Full text @ Nature
Citation
Götz, R., Köster, R.W., Winkler, C., Raulf, F., Lottspeich, F., Schartl, M., and Thoenen, H. (1994) Neurotrophin-6 is a new member of the nerve growth factor family. Nature. 372(6503):266-269.
Abstract
During vertebrate development, many neurons depend for survival and differentiation on their target cells. The best documented mediator of such a retrograde trophic action is the neurotrophin nerve growth factor (NGF). NGF and the other known members of the neurotrophin family, brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5) are conserved as distinct genes over large evolutionary distances. Here we report the cloning of neurotrophin-6 (NT-6), a new member of this family from the teleost fish Xiphophorus. NT-6 distinguishes itself from the other known neurotrophins in that it is not found as a soluble protein in the medium of producing cells. The addition of heparin (but not chondroitin) effects the release of NT-6 from cell surface and extracellular matrix molecules. Recombinant purified NT-6 has a spectrum of actions similar to NGF on chick sympathetic and sensory neurons, albeit with a lower potency. NT-6 is expressed in the embryonic valvulla cerebelli; expression persists in some adult tissues. The interaction of NT-6 with heparin-binding molecules may modulate its action in the nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping