ZFIN ID: ZDB-PUB-060302-6
Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs
Giraldez, A.J., Mishima, Y., Rihel, J., Grocock, R.J., Van Dongen, S., Inoue, K., Enright, A.J., and Schier, A.F.
Date: 2006
Source: Science (New York, N.Y.)   312(5770): 75-79 (Journal)
Registered Authors: Giraldez, Antonio, Inoue, Kunio, Mishima, Yuichiro, Rihel, Jason, Schier, Alexander
Keywords: none
Microarrays: GEO:GSE4201
MeSH Terms:
  • 3' Untranslated Regions
  • Adenosine/metabolism*
  • Animals
  • Embryo, Nonmammalian/physiology*
  • Embryonic Development*
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental*
  • Genes, Reporter
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • MicroRNAs/physiology*
  • Mutation
  • Oligonucleotide Array Sequence Analysis
  • Polymers/metabolism*
  • Protein Biosynthesis
  • RNA Stability
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism*
  • Ribonuclease III/genetics
  • Ribonuclease III/metabolism
  • Transcription, Genetic
  • Up-Regulation
  • Zebrafish/embryology
  • Zygote/physiology
PubMed: 16484454 Full text @ Science
MicroRNAs comprise 1-3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. Using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target mRNAs. Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.