PUBLICATION

Hedgehog signalling from the zona limitans intrathalamica orchestrates patterning of the zebrafish diencephalon

Authors
Scholpp, S., Wolf, O., Brand, M., and Lumsden, A.
ID
ZDB-PUB-060207-11
Date
2006
Source
Development (Cambridge, England)   133(5): 855-864 (Journal)
Registered Authors
Brand, Michael, Lumsden, Andrew, Scholpp, Steffen
Keywords
Regionalization, Forebrain, Shh, Zebrafish, ZLI
MeSH Terms
  • Thalamus/cytology
  • Thalamus/embryology
  • Thalamus/metabolism
  • Diencephalon/cytology
  • Diencephalon/embryology*
  • Diencephalon/metabolism
  • Gene Expression
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Transcriptional Activation
  • Wnt Proteins/metabolism
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Hedgehog Proteins
  • Mutation
  • Body Patterning*/genetics
  • Zebrafish Proteins
  • Trans-Activators/genetics
  • Trans-Activators/metabolism*
  • Animals
  • Signal Transduction
(all 22)
PubMed
16452095 Full text @ Development
Abstract
Midway between the anterior neural border and the midbrain-hindbrain boundary, two well-known local signalling centres in the early developing brain, is a further transverse boundary with putative signalling properties - the zona limitans intrathalamica (ZLI). Here, we describe formation of the ZLI in zebrafish in relation to expression of sonic hedgehog (shh) and tiggy-winkle hedgehog (twhh), and to development of the forebrain regions that flank the ZLI: the prethalamus and thalamus. We find that enhanced Hh signalling increases the size of prethalamic and thalamic gene expression domains, whereas lack of Hh signalling leads to absence of these domains. In addition, we show that shh and twhh display both unique and redundant functions during diencephalic patterning. Genetic ablation of the basal plate shows that Hh expression in the ZLI alone is sufficient for diencephalic differentiation. Furthermore, acquisition of correct prethalamic and thalamic gene expression is dependent on direct Hh signalling. We conclude that proper maturation of the diencephalon requires ZLI-derived Hh signalling.
Genes / Markers
Figures
Figure Gallery (7 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    		Point Mutation
    sb15TgTransgenic Insertion
      tbx392
        		Point Mutation
        tz257
          		Point Mutation
          1 - 4 of 4
          Show
          Human Disease / Model
          Sequence Targeting Reagents
          Fish
          Antibodies
          Orthology
          Engineered Foreign Genes
          No data available
          Mapping
          Your Input Welcome
          We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
          Citation
          Scholpp, S., Wolf, O., Brand, M., and Lumsden, A. (2006) Hedgehog signalling from the zona limitans intrathalamica orchestrates patterning of the zebrafish diencephalon. Development (Cambridge, England). 133(5):855-864.