PUBLICATION

Identification of two proopiomelanocortin genes in zebrafish (Danio rerio)

Authors
Gonzalez-Nuñez, V., Gonzalez-Sarmiento, R., and Rodriguez, R.E.
ID
ZDB-PUB-051214-6
Date
2003
Source
Brain research. Molecular brain research   120(1): 1-8 (Journal)
Registered Authors
González Nuñez, Veronica, Rodriguez, Raquel E.
Keywords
Zebrafish; Opioids; Proopiomelanocortin; beta-Endorphin
MeSH Terms
  • Adrenocorticotropic Hormone/genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain/metabolism
  • Cloning, Molecular
  • Conserved Sequence
  • Exons
  • Introns
  • Phylogeny
  • Pituitary Gland/metabolism
  • Pro-Opiomelanocortin/biosynthesis
  • Pro-Opiomelanocortin/classification
  • Pro-Opiomelanocortin/genetics*
  • RNA, Messenger/biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction/methods
  • Sequence Alignment
  • Zebrafish
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics*
  • alpha-MSH/genetics
  • beta-Endorphin/genetics
  • beta-Lipotropin/genetics
PubMed
14667571 Full text @ Brain Res. Mol. Brain Res.
Abstract
Characterization of a newly cloned proopiomelanocortin (POMC) gene in the teleost zebrafish, Danio rerio, is reported. This gene is formed by three exons and two introns, and its complete cDNA codes for a polypeptide of 222 amino acids. Zebrafish proopiomelanocortin (zfPOMC) contains the consensus sequences for ACTH, gamma-LPH, beta-MSH and beta-endorphin (beta-END). RT-PCR expression studies indicate that zfPOMC is selectively expressed in nervous tissue and in the pituitary gland. An homologous sequence to zfPOMC in the zebrafish genome is also presented. It is possible that this sequence represents part of a duplicate POMC, which might have appeared as a result of an extra genome duplication that have taken place in the cyprinidae family or even in all teleosts. Comparisons between the two zebrafish beta-endorphins and among these peptides and its homologues in other species are also presented.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping