Characterization and expression analysis of TNF-related apoptosis inducing ligand (TRAIL) in grass carp Ctenopharyngodon idella

Chang, M.X., Nie, P., Xie, H.X., Wang, G.L., and Gao, Y.
Veterinary Immunology and Immunopathology   110(1-2): 51-63 (Journal)
Registered Authors
Nie, Pin
TRAIL, Genome, Promoter, Expression, Grass carp
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Apoptosis Regulatory Proteins/biosynthesis*
  • Apoptosis Regulatory Proteins/genetics
  • Apoptosis Regulatory Proteins/immunology
  • Base Sequence
  • Carps/genetics
  • Carps/immunology
  • Carps/metabolism*
  • Cloning, Molecular
  • DNA, Complementary/genetics
  • Membrane Glycoproteins/biosynthesis*
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/immunology
  • Molecular Sequence Data
  • Phylogeny
  • Promoter Regions, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction/veterinary
  • Sequence Alignment
  • Sequence Analysis, DNA
  • TNF-Related Apoptosis-Inducing Ligand
  • Tumor Necrosis Factor-alpha/biosynthesis*
  • Tumor Necrosis Factor-alpha/genetics
  • Tumor Necrosis Factor-alpha/immunology
16216337 Full text @ Vet. Immunol. Immunopathol.
TRAIL (Apo2 ligand) described as a type II transmembrane protein belonging to the TNF superfamily can induce apoptotic cell death in a variety of cell types. In the present study, a putative cDNA sequence encoding the 299 amino acids of TRAIL (GC-TRAIL) and its genomic organization were identified in grass carp Ctenopharyngodon idella. The predicted GC-TRAIL sequence showed 44 and 41% identities to chicken and human TRAILs, respectively. In a domain search, a tumor necrosis factor homology domain (THD) was identified in the C-terminal portion of TRAILs. The GC-TRAIL gene consists of five exons, with four intervening introns, spaced over approximately 4kb of genomic sequence. Analysis of GC-TRAIL promoter region revealed the presence of a number of putative transcription factor binding sites, such as Sp1, NF-kappaB, AP-1, GATA, NFAT, HNF, STAT, P53 and IRF1 sequences which are important for the expression of other TNF family members. Phylogenetic analysis placed GC-TRAIL and the putative zebrafish (Danio rerio) TRAIL obtained from searching the zebrafish database into one separate cluster near mammalian TRAIL genes, but apart from the reported zebrafish TRAIL-like protein, indicating that the GC-TRAIL is an authentic fish TRAIL. Expression analysis revealed that GC-TRAIL is expressed in many tissues, such as in gills, liver, trunk kidney, head kidney, intestine and spleen.
Genes / Markers
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Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes