PUBLICATION

Gata4 regulates the formation of multiple organs

Authors
Holtzinger, A., and Evans, T.
ID
ZDB-PUB-050810-6
Date
2005
Source
Development (Cambridge, England)   132(17): 4005-4014 (Journal)
Registered Authors
Evans, Todd
Keywords
Organogenesis, Heart, Liver, Pancreas, Zebrafish
MeSH Terms
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics*
  • DNA-Binding Proteins/metabolism*
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Transcription Factors/deficiency
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression
  • Gastrointestinal Tract/embryology
  • Gastrointestinal Tract/metabolism
  • GATA6 Transcription Factor
  • GATA4 Transcription Factor
  • Organogenesis*/genetics
  • Heart/embryology
  • Myocardium/metabolism
  • Models, Biological
  • Animals
(all 22)
PubMed
16079152 Full text @ Development
Abstract
We have developed a loss-of-function model for Gata4 in zebrafish, in order to examine broadly its requirement for organogenesis. We show that the function of Gata4 in zebrafish heart development is well conserved with that in mouse, and that, in addition, Gata4 is required for development of the intestine, liver, pancreas and swim bladder. Therefore, a single transcription factor regulates the formation of many organs. Gata6 is a closely related transcription factor with an overlapping expression pattern. We show that zebrafish depleted of Gata6 show defects in liver bud growth similar to mouse Gata6 mutants and zebrafish Gata4 morphants, and that zebrafish embryos depleted of both Gata4 and Gata6 display an earlier block in liver development, and thus completely lack liver buds. Therefore, Gata4 and Gata6 have distinct non-redundant functions in cardiac morphogenesis, but are redundant for an early step of liver development. In addition, both Gata4 and Gata6 are essential and non-redundant for liver growth following initial budding.
Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
ela2lWTstandard conditionsDay 4RTPCR
ela2lWT + MO1-gata4standard conditionsDay 4Not DetectedRTPCR
fabp2WTstandard conditionsDay 4RTPCR
fabp2WT + MO1-gata4standard conditionsDay 4Not DetectedRTPCR
foxa2WTstandard conditionsLong-pecRTPCR
foxa2WTstandard conditionsPrim-5RTPCR
foxa2WT + MO1-gata4standard conditionsPrim-5RTPCR
foxa2WT + MO1-gata4standard conditionsLong-pecRTPCR
gata4WTstandard conditionsLong-pecISH
gata4WTstandard conditionsPrim-5ISH
1 - 10 of 56
Show
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ae5TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    gata4MO1-gata4MRPHLNO
    gata4MO2-gata4MRPHLNO
    gata6MO1-gata6MRPHLNO
    1 - 3 of 3
    Show
    Fish
    Fish
    ae5Tg
    WT
    WT + MO1-gata4
    WT + MO1-gata4 + MO1-gata6
    WT + MO1-gata6
    1 - 5 of 5
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Holtzinger, A., and Evans, T. (2005) Gata4 regulates the formation of multiple organs. Development (Cambridge, England). 132(17):4005-4014.