Retinoic acid signalling links left-right asymmetric patterning and bilaterally symmetric somitogenesis in the zebrafish embryo
- Kawakami, Y., Raya, A., Raya, R.M., Rodriguez-Esteban, C., and Izpisúa Belmonte, J.C.
- Nature 435(7039): 165-171 (Journal)
- Registered Authors
- Izpisúa Belmonte, Juan Carlos, Kawakami, Yasuhiko, Raya, Angel, Raya, Marina, Rodriguez-Esteban, Concepcion
- MeSH Terms
- Body Patterning/physiology*
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism*
- Models, Biological
- Molecular Sequence Data
- Signal Transduction*
- 15889082 Full text @ Nature
Kawakami, Y., Raya, A., Raya, R.M., Rodriguez-Esteban, C., and Izpisúa Belmonte, J.C. (2005) Retinoic acid signalling links left-right asymmetric patterning and bilaterally symmetric somitogenesis in the zebrafish embryo. Nature. 435(7039):165-171.
During embryogenesis, cells are spatially patterned as a result of highly coordinated and stereotyped morphogenetic events. In the vertebrate embryo, information on laterality is conveyed to the node, and subsequently to the lateral plate mesoderm, by a complex cascade of epigenetic and genetic events, eventually leading to a left-right asymmetric body plan. At the same time, the paraxial mesoderm is patterned along the anterior-posterior axis in metameric units, or somites, in a bilaterally symmetric fashion. Here we characterize a cascade of laterality information in the zebrafish embryo and show that blocking the early steps of this cascade (before it reaches the lateral plate mesoderm) results in random left-right asymmetric somitogenesis. We also uncover a mechanism mediated by retinoic acid signalling that is crucial in buffering the influence of the flow of laterality information on the left-right progression of somite formation, and thus in ensuring bilaterally symmetric somitogenesis.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes