PUBLICATION
Development of oxygen sensing in the gills of zebrafish
- Authors
- Jonz, M.G., and Nurse, C.A.
- ID
- ZDB-PUB-050404-9
- Date
- 2005
- Source
- The Journal of experimental biology 208(Pt.8): 1537-1549 (Journal)
- Registered Authors
- Jonz, Michael G.
- Keywords
- O2 chemoreceptor, development, gill, hypoxia, neuroepithelial cells, zebrafish, Danio rerio
- MeSH Terms
-
- Analysis of Variance
- Animals
- Chemoreceptor Cells/embryology
- Chemoreceptor Cells/physiology*
- Dose-Response Relationship, Drug
- Gills/embryology*
- Gills/innervation
- Gills/physiology
- Microscopy, Fluorescence
- Neuroepithelial Cells/cytology
- Neuroepithelial Cells/physiology*
- Oxygen/physiology*
- Oxygen Consumption/drug effects
- Quinidine/pharmacology
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 15802677 Full text @ J. Exp. Biol.
Citation
Jonz, M.G., and Nurse, C.A. (2005) Development of oxygen sensing in the gills of zebrafish. The Journal of experimental biology. 208(Pt.8):1537-1549.
Abstract
Previous studies have described the morphology, innervation and O(2)-chemoreceptive properties of neuroepithelial cells (NECs) of the zebrafish gill filaments. The present work describes the ontogenesis of these cells, and the formation of functional O(2)-sensing pathways in developing zebrafish. Confocal immunofluorescence was performed on whole-mount gill preparations using antibodies against serotonin (5-HT) and a zebrafish-derived neuronal marker (zn-12) to identify the appearance and innervation of gill NECs during larval stages. NECs were first expressed in gill filament primordia of larvae at 5 days postfertilization (d.p.f.) and were fully innervated by 7 d.p.f. In vivo ventilation frequency analysis revealed that a behavioural response to hypoxia (11.2+/-2.8 min(-1)) developed in embryos as early as 2 d.p.f., and a significant increase (P<0.05) in the ventilatory response to hypoxia (200.8+/-23.0 min(-1)) coincided with innervation of NECs of the filaments. In addition, exogenous application of quinidine, a blocker of O(2)-sensitive background K(+) channels in NECs, induced hyperventilation in adults in a dose-dependent manner and revealed the development of a quinidine-sensitive ventilatory response in 7 d.p.f. larvae. This study shows that NEC innervation in the gill filaments may account for the development of a functional O(2)-sensing pathway and the hyperventilatory response to hypoxia in zebrafish larvae. At earlier stages, however, O(2)-sensing must occur through another pathway. The possibility that a new type of 5-HT-positive NEC of the gill arches may account for this earlier hypoxic response is discussed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping