PUBLICATION
Modulation of zebrafish pitx3 expression in the primordia of the pituitary, lens, olfactory epithelium and cranial ganglia by hedgehog and nodal signaling
- Authors
- Zilinski, C.A., Shah, R., Lane, M.E., and Jamrich, M.
- ID
- ZDB-PUB-050113-7
- Date
- 2005
- Source
- Genesis (New York, N.Y. : 2000) 41(1): 33-40 (Journal)
- Registered Authors
- Jamrich, Milan, Lane, Mary Ellen
- Keywords
- none
- MeSH Terms
-
- Amino Acid Motifs
- Amino Acid Sequence
- Animals
- Embryo, Nonmammalian
- Embryonic Development
- Ganglia/cytology
- Ganglia/embryology*
- Ganglia/metabolism
- Gene Expression Regulation, Developmental
- Head
- Hedgehog Proteins
- Homeodomain Proteins/chemistry
- Homeodomain Proteins/metabolism*
- In Situ Hybridization
- Lens, Crystalline/cytology
- Lens, Crystalline/embryology*
- Lens, Crystalline/metabolism
- Molecular Sequence Data
- Morphogenesis
- Mutation
- Nodal Protein
- Olfactory Mucosa/cytology
- Olfactory Mucosa/embryology*
- Olfactory Mucosa/metabolism
- Phylogeny
- Pituitary Gland, Anterior/cytology
- Pituitary Gland, Anterior/embryology*
- Pituitary Gland, Anterior/metabolism
- Protein Structure, Tertiary
- Sequence Homology, Amino Acid
- Trans-Activators/genetics
- Trans-Activators/metabolism*
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism*
- Zebrafish/embryology
- Zebrafish/metabolism
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/metabolism*
- PubMed
- 15645439 Full text @ Genesis
Citation
Zilinski, C.A., Shah, R., Lane, M.E., and Jamrich, M. (2005) Modulation of zebrafish pitx3 expression in the primordia of the pituitary, lens, olfactory epithelium and cranial ganglia by hedgehog and nodal signaling. Genesis (New York, N.Y. : 2000). 41(1):33-40.
Abstract
In this article we report the isolation of a novel zebrafish gene, pitx3, which plays an important role in the formation of several placode-derived structures. In wildtype embryos, pitx3 is first expressed in a crescent-shaped area in the anterior end of the embryo. At later stages, the primordia of the anterior pituitary, the lens, the olfactory sensory epithelium, and cranial ganglia express this gene. Pitx3 is not expressed in the more posterior preplacodal region that gives rise to the epibranchial, otic, and lateral line placodes. The dynamics of pitx3 in the anterior region of wildtype embryos suggests that pitx3 expression marks a common step in the formation of the pituitary, lens, olfactory placode as well as the trigeminal placode. Analysis of pitx3 expression in mutants lacking the hedgehog or nodal function demonstrates the differential dependence of pitx3 expression in these structures on nodal and hedgehog signaling. While the lens and trigeminal placodes express pitx3 in the absence of hedgehog and nodal signaling, there is no expression of pitx3 in the anteriormost ectoderm adjacent to the neural plate from which the anterior pituitary would derive. In mutants with impaired hedgehog signaling, the lens placode frequently extends into more anterior ventral regions of the embryo. genesis 41:33-40, 2005. (c) 2005 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping