ZFIN ID: ZDB-PUB-041216-9
Combined haploid and insertional mutation screen in the zebrafish
Wiellette, E., Grinblat, Y., Austen, M., Hirsinger, E., Amsterdam, A., Walker, C., Westerfield, M., and Sive, H.
Date: 2004
Source: Genesis (New York, N.Y. : 2000)   40(4): 231-240 (Journal)
Registered Authors: Amsterdam, Adam, Grinblat, Yevgenya, Hirsinger, Estelle, Sive, Hazel, Walker, Charline, Westerfield, Monte, Wiellette, Elizabeth
Keywords: none
MeSH Terms:
  • Alleles
  • Animals
  • Body Patterning
  • Brain/embryology
  • Cloning, Molecular
  • Female
  • Guanylate Cyclase/genetics
  • Haploidy*
  • Male
  • Models, Biological
  • Mutagenesis, Insertional/methods*
  • Mutation*
  • Phenotype
  • Somites
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed: 15593329 Full text @ Genesis
To identify genes required for development of the brain and somites, we performed a pilot screen of gynogenetic haploid zebrafish embryos produced from mothers mutagenized by viral insertion. We describe an efficient method to identify new mutations and the affected gene. In addition, we report the results of a small-scale screen that identified five genes required for brain development, including novel alleles of nagie oko, pou5f1, ribosomal protein L36, and n-cadherin, as well as a novel allele of the laminin g1 gene that is required for normal skeletal muscle fiber organization and somite patterning.