PUBLICATION

Combined haploid and insertional mutation screen in the zebrafish

Authors
Wiellette, E., Grinblat, Y., Austen, M., Hirsinger, E., Amsterdam, A., Walker, C., Westerfield, M., and Sive, H.
ID
ZDB-PUB-041216-9
Date
2004
Source
Genesis (New York, N.Y. : 2000)   40(4): 231-240 (Journal)
Registered Authors
Amsterdam, Adam, Grinblat, Yevgenya, Hirsinger, Estelle, Sive, Hazel, Walker, Charline, Westerfield, Monte, Wiellette, Elizabeth
Keywords
none
MeSH Terms
  • Alleles
  • Animals
  • Body Patterning
  • Brain/embryology
  • Cloning, Molecular
  • Female
  • Guanylate Cyclase/genetics
  • Haploidy*
  • Male
  • Models, Biological
  • Mutagenesis, Insertional/methods*
  • Mutation*
  • Phenotype
  • Somites
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed
15593329 Full text @ Genesis
Abstract
To identify genes required for development of the brain and somites, we performed a pilot screen of gynogenetic haploid zebrafish embryos produced from mothers mutagenized by viral insertion. We describe an efficient method to identify new mutations and the affected gene. In addition, we report the results of a small-scale screen that identified five genes required for brain development, including novel alleles of nagie oko, pou5f1, ribosomal protein L36, and n-cadherin, as well as a novel allele of the laminin g1 gene that is required for normal skeletal muscle fiber organization and somite patterning.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes