ZFIN ID: ZDB-PUB-040721-7
Combinatorial Fgf and Bmp signalling patterns the gastrula ectoderm into prospective neural and epidermal domains
Kudoh, T., Concha, M.L., Houart, C., Dawid, I.B., and Wilson, S.W.
Date: 2004
Source: Development (Cambridge, England)   131(15): 3581-3592 (Journal)
Registered Authors: Concha, Miguel, Dawid, Igor B., Houart, Corinne, Kudoh, Tetsuhiro, Wilson, Steve
Keywords: Zebrafish, Default model, Neural induction
MeSH Terms:
  • Animals
  • Biomarkers
  • Body Patterning*
  • Bone Morphogenetic Proteins/antagonists & inhibitors
  • Bone Morphogenetic Proteins/metabolism*
  • Cell Lineage
  • Cell Movement
  • Central Nervous System/embryology*
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Ectoderm/cytology
  • Ectoderm/physiology*
  • Fibroblast Growth Factors/metabolism*
  • Gastrula*/cytology
  • Gastrula*/physiology
  • Gene Expression Regulation, Developmental
  • Glycoproteins/genetics
  • Glycoproteins/metabolism
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/metabolism
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins/genetics
  • Intercellular Signaling Peptides and Proteins/metabolism
  • Organizers, Embryonic
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • SOXB1 Transcription Factors
  • Signal Transduction/physiology*
  • Transcription Factors
  • Zebrafish/embryology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed: 15262889 Full text @ Development
Studies in fish and amphibia have shown that graded Bmp signalling activity regulates dorsal-to-ventral (DV) patterning of the gastrula embryo. In the ectoderm, it is thought that high levels of Bmp activity promote epidermal development ventrally, whereas secreted Bmp antagonists emanating from the organiser induce neural tissue dorsally. However, in zebrafish embryos, the domain of cells destined to contribute to the spinal cord extends all the way to the ventral side of the gastrula, a long way from the organiser. We show that in vegetal (trunk and tail) regions of the zebrafish gastrula, neural specification is initiated at all DV positions of the ectoderm in a manner that is unaffected by levels of Bmp activity and independent of organiser-derived signals. Instead, we find that Fgf activity is required to induce vegetal prospective neural markers and can do so without suppressing Bmp activity. We further show that Bmp signalling does occur within the vegetal prospective neural domain and that Bmp activity promotes the adoption of caudal fate by this tissue.