PUBLICATION
Expression patterns of three estrogen receptor genes during zebrafish (Danio rerio) development: evidence for high expression in neuromasts
- Authors
- Tingaud-Sequeira, A., André, M., Forgue, J., Barthe, C., and Babin, P.J.
- ID
- ZDB-PUB-040721-5
- Date
- 2004
- Source
- Gene expression patterns : GEP 4(5): 561-568 (Journal)
- Registered Authors
- Babin, Patrick J.
- Keywords
- Estrogen receptor, ER alpha, ER beta, esr1, esr2, Estradiol receptor, Hair cell, Supporting cells, Zebrafish, Danio rerio, Larva, Neuromast, In situ hybridization, Lateral line, RNA expression pattern, Epidermis, Brain, Pectoral fin, Branchial arches, Anal papilla, Development, Mechanoreceptive system, Ototoxicity, Quantitative real-time PCR, Maternal factors
- MeSH Terms
-
- Animals
- DNA Primers
- DNA, Complementary/genetics
- Embryo, Nonmammalian/metabolism
- Gene Expression*
- Gene Expression Regulation, Developmental
- Histological Techniques
- In Situ Hybridization
- Neuroepithelial Cells/metabolism*
- Receptors, Estrogen/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology*
- Zebrafish/metabolism*
- PubMed
- 15261834 Full text @ Gene Expr. Patterns
Citation
Tingaud-Sequeira, A., André, M., Forgue, J., Barthe, C., and Babin, P.J. (2004) Expression patterns of three estrogen receptor genes during zebrafish (Danio rerio) development: evidence for high expression in neuromasts. Gene expression patterns : GEP. 4(5):561-568.
Abstract
The estrogen receptor (ER) genes encode a group of nuclear enhancer proteins, which are important ligand-activated transcription factors, modulating estrogen-target gene transcription. In this study we analyzed expression patterns of three zebrafish ER genes, esr1, esr2a, and esr2b, during development using whole-mount in situ hybridization. High levels of esr2a and esr2b of maternal origin are inherited and segregated to the blastomers. After the mid-blastula transition, the three genes exhibit similar spatio-temporal patterns of expression. In 24 h postfertilization (hpf) embryos, high levels of esr2a and esr2b and low levels of esr1 mRNAs are detected in the epidermis, pectoral fin buds, hatching gland and, to a lesser extent, developing brain. From 24hpf onward, the expression of the three genes is down-regulated in the epidermis. By 60hpf, esr2a mRNA is abundant in mature primary neuromasts of the anterior line system and by 3 days postfertilization (dpf), all mature primary neuromasts in both the anterior and posterior lateral line systems express significant levels of esr2a and esr2b transcripts. Histological sections show a high level of esr2a transcripts in both mechanoreceptive hair cells and supporting cells. The transcripts are still detected after neomycin-induced hair cell death, consistent with the presence of esr2a transcripts in supporting cells. From 6dpf onward, esr2a and esr2b transcripts are robustly co-expressed in primary neuromasts, branchial arches, pectoral fins, and anal papilla, while slight labeling is observed for esr1 transcripts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping