PUBLICATION
Metal uptake in zebrafish embryo-larvae exposed to metal-contaminated sediments
- Authors
- Li, W.H., Chan, P.C., and Chan, K.M.
- ID
- ZDB-PUB-040609-14
- Date
- 2004
- Source
- Marine Environmental Research 58(2-5): 829-832 (Journal)
- Registered Authors
- Chan, King-Ming
- Keywords
- Coastal sediment, Embryo-larvae, Heavy metals, Bioaccumulation
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Environmental Pollutants/analysis
- Environmental Pollutants/pharmacokinetics*
- Environmental Pollutants/toxicity
- Enzyme Induction/drug effects
- Geologic Sediments/analysis*
- Hong Kong
- Larva/metabolism
- Metallothionein/biosynthesis
- Metals, Heavy/analysis
- Metals, Heavy/pharmacokinetics*
- Metals, Heavy/toxicity
- Reproduction/drug effects
- Reproduction/physiology
- Reverse Transcriptase Polymerase Chain Reaction
- Spectrophotometry, Atomic
- Zebrafish/metabolism*
- Zebrafish/physiology
- PubMed
- 15178120 Full text @ Mar. Environ. Res.
Citation
Li, W.H., Chan, P.C., and Chan, K.M. (2004) Metal uptake in zebrafish embryo-larvae exposed to metal-contaminated sediments. Marine Environmental Research. 58(2-5):829-832.
Abstract
Heavy metal contamination in coastal and estuarine regions of inner Tolo Harbour, as well as some river and nullah areas, is one of the major water-pollution issues in Hong Kong. Zebrafish (Danio rerio) embryo-larvae was used to study metal uptake from coastal sediments collected from Fo Tan River (industrial area, Sha Tin), Tai Po River (residential area, Tai Po) and Wu Kai Sa (rural area, Tolo Harbour). Exposure experiments (7-days) were carried out using different concentrations (0.1%, 0.5%, 1% and 0% as control) of sediments that were added to aquaria containing fertilized zebrafish eggs until they hatched to become larvae. Uptake of heavy metals (Cd, Cu, Pb and Zn) was determined in whole embryo-larvae following exposure. Significant levels of Cd, Cu, Pb and Zn were detected in the embryo-larvae exposed to sediments from Tai Po River. However, significant levels of only Cd and Cu were found in embryo-larvae exposed to sediments from Fo Tan River.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping