ZFIN ID: ZDB-PUB-040505-1
In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish
Langenau, D.M., Ferrando, A.A., Traver, D., Kutok, J.L., Hezel, J.P., Kanki, J.P., Zon, L.I., Look, A.T., and Trede, N.S.
Date: 2004
Source: Proceedings of the National Academy of Sciences of the United States of America   101(19): 7369-7374 (Journal)
Registered Authors: Kanki, John, Langenau, David, Look, A. Thomas, Traver, David, Trede, Nick, Zon, Leonard I.
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/immunology*
  • Dexamethasone/pharmacology
  • Immunohistochemistry
  • In Situ Hybridization
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes/drug effects
  • T-Lymphocytes/immunology*
  • Zebrafish/immunology*
PubMed: 15123839 Full text @ Proc. Natl. Acad. Sci. USA
Transgenic zebrafish that express GFP under control of the T cell-specific tyrosine kinase (lck) promoter were used to analyze critical aspects of the immune system, including patterns of T cell development and T cell homing after transplant. GFP-labeled T cells could be ablated in larvae by either irradiation or dexamethasone added to the water, illustrating that T cells have evolutionarily conserved responses to chemical and radiation ablation. In transplant experiments, thymocytes from lck-GFP fish repopulated the thymus of irradiated wild-type fish only transiently, suggesting that the thymus contains only short-term thymic repopulating cells. By contrast, whole kidney marrow permanently reconstituted the T lymphoid compartment of irradiated wild-type fish, suggesting that long-term thymic repopulating cells reside in the kidney.