ZFIN ID: ZDB-PUB-040402-3
Her5 acts as a prepattern factor that blocks neurogenin1 and coe2 expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary
Geling, A., Plessy, C., Rastegar, S., Strähle, U., and Bally-Cuif, L.
Date: 2004
Source: Development (Cambridge, England)   131(9): 1993-2006 (Journal)
Registered Authors: Bally-Cuif, Laure, Geling, Andrea, Plessy, Charles, Rastegar, Sepand, Strähle, Uwe
Keywords: Her5, Hairy, Midbrain-hindbrain boundary, Zebrafish, Neurogenesis, Pre-patterning
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Basic Helix-Loop-Helix Transcription Factors
  • Body Patterning*
  • Cell Differentiation/physiology
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Embryonic Structures/anatomy & histology
  • Embryonic Structures/physiology
  • Enhancer Elements, Genetic
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization
  • Membrane Proteins/metabolism
  • Mesencephalon/cytology
  • Mesencephalon/metabolism*
  • Morphogenesis/physiology
  • Neurons/cytology
  • Neurons/metabolism
  • Receptors, Notch
  • Rhombencephalon/cytology
  • Rhombencephalon/metabolism*
  • Signal Transduction/physiology
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Transgenes
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/physiology
  • Zebrafish Proteins/metabolism*
PubMed: 15056616 Full text @ Development
Neurogenesis in both vertebrates and invertebrates is tightly controlled in time and space involving both positive and negative regulators. We report here that the bHLH factor Her5 acts as a prepattern gene to prevent neurogenesis in the anlage of the midbrain/hindbrain boundary in the zebrafish neural plate. This involves selective suppression of both neurogenin1 (ngn1) and coe2 mRNA expression in a process that is independent of Notch signalling, and where inhibition of either ngn1 or coe2 expression is sufficient to prevent neuronal differentiation across the midbrain-hindbrain boundary. A ngn1 transgene faithfully responds to Her5 and deletion analysis of the transgene identifies an E-box in a ngn1 upstream enhancer to be required for repression by Her5. Together our data demonstrate a role of Her5 as a prepattern factor in the spatial definition of proneural domains in the zebrafish neural plate, in a manner similar to its Drosophila homologue Hairy.