ZFIN ID: ZDB-PUB-040109-9
Isolation of nlz2 and characterization of essential domains in Nlz-family proteins
Runko, A.P. and Sagerström, C.G.
Date: 2004
Source: The Journal of biological chemistry   279(12): 11917-11925 (Journal)
Registered Authors: Runko, Alexander, Sagerström, Charles
Keywords: none
MeSH Terms:
  • Base Sequence
  • Blotting, Western
  • Cloning, Molecular
  • DNA Primers
  • Glutathione Transferase/metabolism
  • Molecular Sequence Data
  • Proteins/chemistry
  • Proteins/genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Structure-Activity Relationship
  • Zinc Fingers
PubMed: 14709556 Full text @ J. Biol. Chem.
In this study we first clone nlz2, a second zebrafish member of the nlz-related zinc-finger gene family. nlz2 is expressed together with nlz1 in a broad posterior domain during gastrula stages, as well as at the midbrain-hindbrain boundary and in the hindbrain caudal to rhombomere 4 during segmentation. nlz2 is also expressed in regions distinct from nlz1, notably in the forebrain, midbrain and trunk. Misexpression of nlz2 in zebrafish embryos disrupts gene expression in the rostral hindbrain, similar to the effect of misexpressing nlz1. We next compare the nlz1 and nlz2 sequences to identify and characterize domains conserved within this family. We find a C-terminal domain required for nuclear localization and two conserved domains (the Sp motif and a putative C(2)H(2) zinc finger) required for nlz1 function. We also demonstrate that Nlz1 self-associates via its C-terminus, interacts with Nlz2 and binds to histone deacetylases. Lastly, we find two forms of Nlz1 generated from alternative translation initiation sites in vivo. These forms have distinct activities, apparently depending on function of the N-terminal Sp motif. Our data demonstrate that nlz2 functions similarly to nlz1 and define conserved domains essential for nuclear localization, self-association and corepressor binding in this novel family of zinc-finger genes.