PUBLICATION

Cadherin-1, -2, and -11 expression and cadherin-2 function in the pectoral limb bud and fin of the developing zebrafish

Authors
Liu, Q., Kerstetter, A.E., Azodi, E., and Marrs, J.A.
ID
ZDB-PUB-031204-5
Date
2003
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   228(4): 734-739 (Journal)
Registered Authors
Liu, Qin, Marrs, James A.
Keywords
zebrafish embryos, cell adhesion molecules, development, pectoral fins
MeSH Terms
  • Animals
  • Cadherins/biosynthesis*
  • Cadherins/physiology*
  • Extremities/embryology*
  • Gene Expression Regulation, Developmental*
  • Immunohistochemistry
  • In Situ Hybridization
  • Limb Buds/embryology*
  • Oligonucleotides, Antisense/pharmacology
  • RNA, Messenger/metabolism
  • Zebrafish
PubMed
14648850 Full text @ Dev. Dyn.
Abstract
Cadherins are cell adhesion molecules that play important roles in development of a variety of organs, including the vertebrate limb. In this study, we analyze cadherin expression patterns in the embryonic zebrafish pectoral limb buds and larval pectoral fins by using both in situ hybridization and immunocytochemical methods. cadherin-1 is detected in the epidermis of the embryonic limb buds and the larval pectoral fins. Cadherin-2 is expressed in the pectoral limb bud mesenchyme and chondrogenic condensation. As development proceeds, cadherin-2 expression is detected in newly differentiated pectoral fin endoskeleton, but its expression is greatly down-regulated in the fin endoskeleton of larval zebrafish. cadherin-11 is found in the basal region of the embryonic limb buds and in the proximal endoskeleton of the larval pectoral fins. Interfering with cadherin-2 function using two specific antisense morpholino oligonucleotides disrupts formation of the chondrogenic condensation/endoskeleton, suggesting that cadherin-2 is crucial for the normal development of the zebrafish pectoral fins.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping