PUBLICATION
Molecular cloning and tissue-specific, developmental-stage-specific, and hormonal regulation of IGFBP3 gene in zebrafish
- Authors
- Chen, J.Y., Chen, J.C., Huang, W.T., Liu, C.W., Hui, C.F., Chen, T.T., and Wu, J.L.
- ID
- ZDB-PUB-031111-5
- Date
- 2004
- Source
- Marine biotechnology (New York, N.Y.) 6(1): 1-7 (Journal)
- Registered Authors
- Chen, Jyh-Yih, Huang, Wei-Tung, Wu, Jen-Leih
- Keywords
- cloning, zebrafish, IGFBP3, hormone regulation
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- DNA Primers
- DNA, Complementary/genetics
- Female
- Food Deprivation/physiology
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/physiology*
- Insulin/pharmacology
- Insulin-Like Growth Factor Binding Protein 3/genetics*
- Insulin-Like Growth Factor Binding Protein 3/metabolism
- Molecular Sequence Data
- Ovary/metabolism
- RNA, Messenger/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Alignment
- Sequence Analysis, DNA
- Somatomedins/pharmacology
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 14605935 Full text @ Mar. Biotechnol.
Citation
Chen, J.Y., Chen, J.C., Huang, W.T., Liu, C.W., Hui, C.F., Chen, T.T., and Wu, J.L. (2004) Molecular cloning and tissue-specific, developmental-stage-specific, and hormonal regulation of IGFBP3 gene in zebrafish. Marine biotechnology (New York, N.Y.). 6(1):1-7.
Abstract
The biological functions of insulin-like growth factor (IGF) I and II are modulated by a family of IGF-binding proteins (IGFBPs) in complex IGF-dependent and IGF-independent pathways. For further understanding of the actions of IGFs, some of these binding proteins have been cloned and characterized. We report the molecular cloning of IGFBP-3 cDNA for zebrafish. The tissue-specific and developmental stage-specific expression of IGFBP-3 and the hormonal regulation of its expression have also been determined by comparative reverse transcription polymerase chain reaction. Zebrafish IGFBP-3 cDNA contains an open reading frame of 879 bp, encoding a polypeptide of 293 amino acid residues. Results of this analysis revealed high levels of IGFBP-3 messenger RNA in ovary and fin tissue. Expression of IGFBP-3 mRNA was throughout the entire embryonic development, with the highest level of expression observed at 36 hours after the onset of development. Elevated levels of expression of IGFBP-3 were observed 24 hours after injection with IGF-I and 48 hours with IGF-II or insulin. These results suggest that the expression of IGFBP3 gene might be modulated by IGF-I, IGF-II, and insulin.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping