ZFIN ID: ZDB-PUB-031111-5
Molecular cloning and tissue-specific, developmental-stage-specific, and hormonal regulation of IGFBP3 gene in zebrafish
Chen, J.Y., Chen, J.C., Huang, W.T., Liu, C.W., Hui, C.F., Chen, T.T., and Wu, J.L.
Date: 2004
Source: Marine biotechnology (New York, N.Y.)   6(1): 1-7 (Journal)
Registered Authors: Chen, Jyh-Yih, Huang, Wei-Tung, Wu, Jen-Leih
Keywords: cloning, zebrafish, IGFBP3, hormone regulation
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • DNA Primers
  • DNA, Complementary/genetics
  • Female
  • Food Deprivation/physiology
  • Gene Expression Regulation, Developmental/drug effects
  • Gene Expression Regulation, Developmental/physiology*
  • Insulin/pharmacology
  • Insulin-Like Growth Factor Binding Protein 3/genetics*
  • Insulin-Like Growth Factor Binding Protein 3/metabolism
  • Molecular Sequence Data
  • Ovary/metabolism
  • RNA, Messenger/genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Somatomedins/pharmacology
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed: 14605935 Full text @ Mar. Biotechnol.
The biological functions of insulin-like growth factor (IGF) I and II are modulated by a family of IGF-binding proteins (IGFBPs) in complex IGF-dependent and IGF-independent pathways. For further understanding of the actions of IGFs, some of these binding proteins have been cloned and characterized. We report the molecular cloning of IGFBP-3 cDNA for zebrafish. The tissue-specific and developmental stage-specific expression of IGFBP-3 and the hormonal regulation of its expression have also been determined by comparative reverse transcription polymerase chain reaction. Zebrafish IGFBP-3 cDNA contains an open reading frame of 879 bp, encoding a polypeptide of 293 amino acid residues. Results of this analysis revealed high levels of IGFBP-3 messenger RNA in ovary and fin tissue. Expression of IGFBP-3 mRNA was throughout the entire embryonic development, with the highest level of expression observed at 36 hours after the onset of development. Elevated levels of expression of IGFBP-3 were observed 24 hours after injection with IGF-I and 48 hours with IGF-II or insulin. These results suggest that the expression of IGFBP3 gene might be modulated by IGF-I, IGF-II, and insulin.