PUBLICATION
The role of epithelial remodelling in tooth eruption in larval zebrafish
- Authors
- Huysseune, A. and Sire, J.-Y.
- ID
- ZDB-PUB-031031-1
- Date
- 2004
- Source
- Cell and tissue research 315(1): 85-95 (Journal)
- Registered Authors
- Huysseune, Ann, Sire, Jean-Yves
- Keywords
- Tooth eruption, Epithelial morphogenesis, Zebrafish, Danio rerio (Teleostei)
- MeSH Terms
-
- Animals
- Branchial Region*/growth & development*
- Branchial Region*/ultrastructure
- Epithelium/growth & development*
- Tooth Eruption
- Zebrafish/growth & development*
- PubMed
- 14586692 Full text @ Cell Tissue Res.
Citation
Huysseune, A. and Sire, J.-Y. (2004) The role of epithelial remodelling in tooth eruption in larval zebrafish. Cell and tissue research. 315(1):85-95.
Abstract
Based on light and transmission electron-microscopic observations on erupting first-generation teeth in the zebrafish, Danio rerio, we propose a biphasic mechanism for tooth eruption: (1) formation of an epithelial crypt prior to eruption of the tooth, possibly as a result of constraints in the epithelium resulting from the growth of adjacent tooth germs, and (2) detachment of cellular interdigitations both within the pharyngeal epithelium, at the pharyngeal epithelium/enamel organ boundary, and between the outer and inner dental epithelium, resulting in the exposure of the tooth tip in the crypt, immediately after tooth ankylosis. Later, further detachment of interdigitations between the inner and the outer enamel epithelium unfolds the epithelium even more and leads to a more pronounced exposure of the tooth tip. The presence of small patches of non-collagenous matrix on the outer surface of the tooth close to where it merges with the attachment bone is interpreted as a device to prevent complete detachment of the enamel organ. The biphasic nature of the mechanism for tooth eruption is supported by observations on in vitro cultured heads. First-generation teeth develop normally and crypts are formed, as under in vivo conditions, but the teeth fail to erupt. Taken together, our observations suggest that epithelial remodelling plays a crucial role in eruption of the teeth in this model organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping