PUBLICATION
Gonad development and vitellogenin production in zebrafish (Danio rerio) exposed to ethinylestradiol and methyltestosterone
- Authors
- Orn, S., Holbech, H., Madsen, T.H., Norrgren, L., and Petersen, G.I.
- ID
- ZDB-PUB-031022-2
- Date
- 2003
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 65(4): 397-411 (Journal)
- Registered Authors
- Holbech, Henrik, Norrgren, Leif, Petersen, Gitte I.
- Keywords
- Danio rerio, Partial life-cycle test, Endocrine disruptor, Vitellogenin, Sex ratio
- MeSH Terms
-
- Animals
- Disorders of Sex Development
- Dose-Response Relationship, Drug
- Ethinyl Estradiol/pharmacology*
- Female
- Gonads/anatomy & histology
- Gonads/drug effects*
- Gonads/growth & development
- Life Cycle Stages*
- Male
- Methyltestosterone/pharmacology*
- Sex Ratio
- Vitellogenins/biosynthesis
- Vitellogenins/drug effects*
- Zebrafish/growth & development*
- Zebrafish/metabolism*
- PubMed
- 14568354 Full text @ Aquat. Toxicol.
Citation
Orn, S., Holbech, H., Madsen, T.H., Norrgren, L., and Petersen, G.I. (2003) Gonad development and vitellogenin production in zebrafish (Danio rerio) exposed to ethinylestradiol and methyltestosterone. Aquatic toxicology (Amsterdam, Netherlands). 65(4):397-411.
Abstract
In a partial life-cycle test, the impact of 17alpha-ethinylestradiol (EE2) and 17alpha-methyltestosterone (MT) on juvenile zebrafish was evaluated by use of vitellogenin measurements and gonadal development. Exposure to EE2 (1-25ng/l) resulted in a dose-dependent increase in vitellogenin production starting at 2ng/l. Significant changes in sex ratios in female direction were detected at 1ng/l, with complete sex reversal taking place after exposure to 2ng/l. No intersex fish were observed after exposure to EE2. Exposure to MT resulted in decreased vitellogenin concentrations. Complete sex reversal was detected in all MT concentrations used (26-1000ng/l). A large proportion of intersex fish was observed after exposure to 1000ng MT/l. The period of gonadal sex reversal in non-exposed zebrafish was also studied. The main morphological features of the transformation of ovaries into testis were observed 4-5 weeks after hatching.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping