PUBLICATION

The maternal-effect gene futile cycle is essential for pronuclear congression and mitotic spindle assembly in the zebrafish zygote

Authors
Dekens, M.P., Pelegri, F.J., Maischein, H.M., and Nüsslein-Volhard, C.
ID
ZDB-PUB-030728-20
Date
2003
Source
Development (Cambridge, England)   130(17): 3907-3916 (Journal)
Registered Authors
Dekens, Marcus P.S., Maischein, Hans-Martin, Nüsslein-Volhard, Christiane, Pelegri, Francisco
Keywords
none
MeSH Terms
  • Animals
  • Cell Division/physiology*
  • Centrosome/physiology
  • Cleavage Stage, Ovum/cytology
  • Cleavage Stage, Ovum/physiology*
  • Female
  • Genes, cdc*
  • Meiosis/physiology
  • Mitosis/physiology
  • Spindle Apparatus/physiology*
  • Transcription, Genetic/physiology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zygote/cytology
  • Zygote/physiology*
PubMed
12874114 Full text @ Development
Abstract
Embryos have been successfully used for the general study of the cell cycle. Although there are significant differences between the early embryonic and the somatic cell cycle in vertebrates, the existence of specialised factors that play a role during the early cell cycles has remained elusive. We analysed a lethal recessive maternal-effect mutant, futile cycle (fue), isolated in a maternal-effect screen for nuclear division defects in the zebrafish (Danio rerio). The pronuclei fail to congress in zygotes derived from homozygous fue mothers. In addition, a defect in the formation of chromosomal microtubules prevents mitotic spindle assembly and thus chromosome segregation in fue zygotes. However, centrosomal functions do not appear to be affected in fue embryos, suggesting this mutant blocks a subset of microtubule functions. Cleavage occurs normally for several divisions resulting in many anucleate cells, thus showing that nuclear- and cell division can be uncoupled genetically. Therefore, we propose that in mitotic spindle assembly chromosome-dependent microtubule nucleation is essential for the coupling of nuclear and cell division.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes