PUBLICATION

Positional cloning of a temperature-sensitive mutant emmental reveals a role for sly1 during cell proliferation in zebrafish fin regeneration

Authors
Nechiporuk, A., Poss, K.D., Johnson, S.L., and Keating, M.T.
ID
ZDB-PUB-030716-21
Date
2003
Source
Developmental Biology   258(2): 291-306 (Journal)
Registered Authors
Johnson, Stephen L., Keating, Mark T., Nechiporuk, Alex, Poss, Kenneth D.
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Division
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary/genetics
  • Extremities/growth & development
  • Extremities/physiology
  • Female
  • Gene Expression
  • In Situ Hybridization
  • Laminin/genetics
  • Laminin/physiology*
  • Male
  • Molecular Sequence Data
  • Mutation
  • Regeneration*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Temperature
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
12798289 Full text @ Dev. Biol.
Abstract
Here, we used classical genetics in zebrafish to identify temperature-sensitive mutants in caudal fin regeneration. Gross morphological, histological, and molecular analyses revealed that one of these strains, emmental (emm), failed to form a functional regeneration blastema. Inhibition of emm function by heat treatment during regenerative outgrowth rapidly blocked regeneration. This block was associated with reduced proliferation in the proximal blastema and expansion of the nonproliferative distal blastemal zone. Positional cloning revealed that the emm phenotype is caused by a mutation in the orthologue of yeast sly1, a gene product involved in protein trafficking. sly1 is upregulated in the newly formed blastema as well as during regenerative outgrowth. Thus, sly1 is essential for blastemal organization and proliferation during two stages of fin regeneration.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping