PUBLICATION
Comparisons of calcium regulation in fish larvae
- Authors
- Chen, Y.-Y., Lu, F.-I., and Hwang, P.-P.
- ID
- ZDB-PUB-030210-9
- Date
- 2003
- Source
- The Journal of experimental zoology 295A(2): 127-135 (Journal)
- Registered Authors
- Chen, Yi-yen, Hwang, Pung Pung, Lu, Fu-I
- Keywords
- none
- MeSH Terms
-
- Adaptation, Physiological
- Animals
- Calcium/metabolism*
- Calcium/pharmacology
- Dose-Response Relationship, Drug
- Fishes/growth & development*
- Fishes/metabolism*
- Kinetics
- Larva/drug effects
- Larva/metabolism*
- Ovum/metabolism
- Species Specificity
- Time Factors
- Water/chemistry
- PubMed
- 12541296 Full text @ J. Exp. Zool.
Citation
Chen, Y.-Y., Lu, F.-I., and Hwang, P.-P. (2003) Comparisons of calcium regulation in fish larvae. The Journal of experimental zoology. 295A(2):127-135.
Abstract
The purpose of the present study was to compare the ability of larvae of different species, goldfish (Carassius auratus), zebrafish (Danio rerio ), and ayu (Plecoglossus altivelis), to regulate their calcium balance. Whole-body Ca(2+) content and Ca(2+) influx in the larvae of the three species, which were incubated in low- (0.02 mM), mid- (0.2 mM), and high - (2.0 mM) Ca(2+) artificial fresh water from embryonic stages, were compared. The Ca(2+) uptake kinetics were determined in zebrafish and goldfish incubated in high- or low-Ca(2+) artificial fresh water. Ca(2 +) content of both zebrafish and ayu acclimated to low-Ca(2+) media were significantly lower than those acclimated to mid- or high-Ca(2+) media However, Ca(2+) contents of goldfish in low-, mid-, and high-Ca(2+) groups showed no significant differences. In goldfish, Ca(2+) influx in the low-Ca(2+) group was significantly higher than those of the mid- and high-Ca(2+) groups. In contrast, the Ca(2+) influx rate in the low-Ca(2 +) group was significantly lower than those in the mid- and high-Ca(2+) groups in zebrafish and ayu. Compared to the high-Ca(2+) group, the low- Ca(2+) group of goldfish showed a 13% increase in the maximal velocity ( J(max)) and an 84% decrease in the Michaelis constant (K(m)) for Ca(2+) influx. Smaller changes, i.e., an 8% increase in J(max) and a 67% decrease in K(m), were found in zebrafish larvae. Goldfish possess a more effective Ca(2+) regulatory capacity than do zebrafish and ayu. Differences in the strategies for Ca(2+) balance may be associated with different development patterns and environments in which these fish naturally occur.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping