ZFIN ID: ZDB-PUB-030103-7
Introduction of green fluorescent protein and Lac-Z gene into zebrafish (Danio rerio) egg by microinjection and particle gun
Chen, J.-Y., Yang, J.-Y., Wang, J.-I., and Wu, J.-L.
Date: 2001
Source: J. Fish. Soc. Taiwan   28(2): 91-103 (Journal)
Registered Authors: Chen, Jyh-Yih, Wu, Jen-Leih
Keywords: Genes; Biotechnology; Proteins; Fluorescence; Bioluminescence; Danio rerio; Aequorea victoria, Zebra danio; Water jellyfish
MeSH Terms: none
PubMed: none
Green fluorescent protein (GFP) from Aequorea victoria is a novel marker compared with other bioassay markers that has potential use in the study of teleost development biology, transgenic research, and aquaculture. Microinjection and particle gun were used to transfer pCMV- beta into fertilized zebrafish eggs. After staining analysis, the data showed 80% and 6% expression rates, respectively. After microinjection of the pEGFP-C1 vector into fertilized zebrafish eggs, the expression rate was 80%. GFP was visible 5 h after microinjection of the pEGFP-C1 vector. Following advancement of the development stage, the signal was visible in muscle skeletal fibers, heart, and corpuscles of living embryos. Transgenic fish continued to express high levels of GFP in bone marrow, eyes, and muscle in 42-d-old zebrafish as revealed by histological sections data.