PUBLICATION
Introduction of green fluorescent protein and Lac-Z gene into zebrafish (Danio rerio) egg by microinjection and particle gun
- Authors
- Chen, J.-Y., Yang, J.-Y., Wang, J.-I., and Wu, J.-L.
- ID
- ZDB-PUB-030103-7
- Date
- 2001
- Source
- J. Fish. Soc. Taiwan 28(2): 91-103 (Journal)
- Registered Authors
- Chen, Jyh-Yih, Wu, Jen-Leih
- Keywords
- Genes; Biotechnology; Proteins; Fluorescence; Bioluminescence; Danio rerio; Aequorea victoria, Zebra danio; Water jellyfish
- MeSH Terms
- none
- PubMed
- none
Citation
Chen, J.-Y., Yang, J.-Y., Wang, J.-I., and Wu, J.-L. (2001) Introduction of green fluorescent protein and Lac-Z gene into zebrafish (Danio rerio) egg by microinjection and particle gun. J. Fish. Soc. Taiwan. 28(2):91-103.
Abstract
Green fluorescent protein (GFP) from Aequorea victoria is a novel marker compared with other bioassay markers that has potential use in the study of teleost development biology, transgenic research, and aquaculture. Microinjection and particle gun were used to transfer pCMV- beta into fertilized zebrafish eggs. After staining analysis, the data showed 80% and 6% expression rates, respectively. After microinjection of the pEGFP-C1 vector into fertilized zebrafish eggs, the expression rate was 80%. GFP was visible 5 h after microinjection of the pEGFP-C1 vector. Following advancement of the development stage, the signal was visible in muscle skeletal fibers, heart, and corpuscles of living embryos. Transgenic fish continued to express high levels of GFP in bone marrow, eyes, and muscle in 42-d-old zebrafish as revealed by histological sections data.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping