PUBLICATION
Guidance of primordial germ cell migration by the chemokine SDF-1
- Authors
- Doitsidou, M., Reichman-Fried, M., Stebler, J., Köprunner, M., Dörries, J., Meyer, D., Esguerra, C.V., Leung, T., and Raz, E.
- ID
- ZDB-PUB-021202-2
- Date
- 2002
- Source
- Cell 111(5): 647-659 (Journal)
- Registered Authors
- Doitsidou, Maria, Dörries, Julia, Esguerra, Camila V., Köprunner, Marion, Leung, Tin Chung, Meyer, Dirk, Raz, Erez, Reichman-Fried, Michal
- Keywords
- none
- MeSH Terms
-
- Animals
- Body Patterning
- Cell Movement*
- Cell Polarity
- Chemokine CXCL12
- Chemokines, CXC/metabolism*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology
- Gene Expression Regulation, Developmental
- Germ Cells/cytology
- Germ Cells/physiology*
- Mutation
- Oligonucleotides, Antisense/genetics
- Protein Biosynthesis
- RNA, Messenger/metabolism
- Receptors, CXCR4/metabolism*
- Signal Transduction
- Tissue Distribution
- Zebrafish
- PubMed
- 12464177 Full text @ Cell
Citation
Doitsidou, M., Reichman-Fried, M., Stebler, J., Köprunner, M., Dörries, J., Meyer, D., Esguerra, C.V., Leung, T., and Raz, E. (2002) Guidance of primordial germ cell migration by the chemokine SDF-1. Cell. 111(5):647-659.
Abstract
The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping