PUBLICATION
Induction of cranial and posterior trunk neural crest by exogenous retinoic acid in zebrafish
- Authors
- Li, M., Su, Y., and Meng, A.M.
- ID
- ZDB-PUB-021018-8
- Date
- 2002
- Source
- Chinese science bulletin = Kexue tongbao 47(13): 1105-1108 (Journal)
- Registered Authors
- Meng, Anming
- Keywords
- zebrafish, retinoic acid, neural crest, sox9b, crestin
- MeSH Terms
- none
- PubMed
- none
Citation
Li, M., Su, Y., and Meng, A.M. (2002) Induction of cranial and posterior trunk neural crest by exogenous retinoic acid in zebrafish. Chinese science bulletin = Kexue tongbao. 47(13):1105-1108.
Abstract
Retinoic acid (RA) plays an important role in development of vertebrate embryos. We demonstrate impacts of exogenous RA on the formation of neural crest cells in zebrafish using specific neural crest markers sox9b and crestin. Treatment with all-trans RA at 10(-7) mmol/L at 50% epiboly induces sox9b expression in the forebrain and crestin expression in the forebrain and midbrain, resulting in significant increase of pigment cells in the head derived from the cranial neural crest. In addition, RA treatment induces expression of sox9b and crestin in the caudal marginal cells of the neuroectoderm during early segmentation. Earlier commitment of these cells to the neural crest fate in the posterior margins leads to abnormal development of the posterior body, probably by preventing mingling of ventral derived and dorsal-derived cells during the formation of the tailbud.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping