PUBLICATION
Do cardiac neural crest defects in zebrafish result in loss of cardiomyocytes?
- Authors
- Sato, M. and Yost, H.J.
- ID
- ZDB-PUB-021017-67
- Date
- 2002
- Source
- Developmental Biology 247(2): 508 (Abstract)
- Registered Authors
- Yost, H. Joseph
- Keywords
- none
- MeSH Terms
- none
- PubMed
- none
Citation
Sato, M. and Yost, H.J. (2002) Do cardiac neural crest defects in zebrafish result in loss of cardiomyocytes?. Developmental Biology. 247(2):508.
Abstract
Cardiac neural crest is essential for cardiac development in mammals and chick, contributing to conotruncal cushion formation, outflow tract septation, and aortic arch remodeling. We use zebrafish as a tool for studying cardiac neural crest. In zebrafish, we have shown by fate mapping that cardiac neural crest contributes to cardiomyocyte formation as well as pharyngeal arch development. Analyses of several neural crest mutants with heart defects will help us understand the role of cardiac neural crest in cardiac morphogenesis. One of the mutants we are examining is alyron, a recessive, embryonic-lethal mutant that has severe defects of both cranial and trunk neural crest cells. At 24 hpf, alyron embryos have two chambered, beating hearts; severe pericardiac edema; and no blood circulation. We found that expression patterns of neuralcrest-specific genes and cardiomyocyte-specific genes (cmlc2 and vmhc) were altered in alyron mutants. Our results suggest that cardiac neural crest contributes to the formation of the atrium and ventricle in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping