PUBLICATION

Zebrafish smoothened functions in ventral neural tube specification and axon tract formation

Authors
Varga, Z.M., Amores, A., Lewis, K.E., Yan, Y.-L., Postlethwait, J.H., Eisen, J.S., and Westerfield, M.
ID
ZDB-PUB-010927-3
Date
2001
Source
Development (Cambridge, England)   128(18): 3497-3509 (Journal)
Registered Authors
Amores, Angel, Eisen, Judith S., Lewis, Katharine E., Postlethwait, John H., Varga, Zoltan M., Westerfield, Monte, Yan, Yi-Lin
Keywords
hedgehog; forebrain patterning; spinal cord; floor plate; motoneurons; pituitary ventral neural tube; zebrafish
MeSH Terms
  • Animals
  • Body Patterning*
  • Hedgehog Proteins
  • Molecular Sequence Data
  • Motor Neurons
  • Mutation
  • Nervous System/cytology
  • Nervous System/embryology*
  • Phenotype
  • Pituitary Gland, Anterior/cytology
  • Pituitary Gland, Anterior/embryology
  • Prosencephalon/cytology
  • Prosencephalon/embryology
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism*
  • Receptors, G-Protein-Coupled*
  • Retina/cytology
  • Retina/embryology
  • Signal Transduction
  • Spinal Cord/cytology
  • Spinal Cord/embryology
  • Trans-Activators/metabolism
  • Transcription Factors/genetics
  • Visual Pathways/cytology
  • Visual Pathways/embryology
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
PubMed
11566855
Abstract
Sonic hedgehog (Shh) signaling patterns many vertebrate tissues. shh mutations dramatically affect mouse ventral forebrain and floor plate but produce minor defects in zebrafish. Zebrafish have two mammalian Shh orthologs, sonic hedgehog and tiggy-winkle hedgehog, and another gene, echidna hedgehog, that could have overlapping functions. To examine the role of Hedgehog signaling in zebrafish, we have characterized slow muscle omitted (smu) mutants. We show that smu encodes a zebrafish ortholog of Smoothened that transduces Hedgehog signals. Zebrafish smoothened is expressed maternally and zygotically and supports specification of motoneurons, pituitary cells and ventral forebrain. We propose that smoothened is required for induction of lateral floor plate and a subpopulation of hypothalamic cells and for maintenance of medial floor plate and hypothalamic cells.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes