ZFIN ID: ZDB-PUB-010905-2
Critical role of biklf in erythroid cell differentiation in zebrafish
Kawahara, A. and Dawid, I.B.
Date: 2001
Source: Current biology : CB   11(17): 1353-1357 (Journal)
Registered Authors: Dawid, Igor B., Kawahara, Atsuo
Keywords: none
MeSH Terms:
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Differentiation
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Erythroid Precursor Cells/cytology*
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • Globins/genetics
  • Globins/metabolism
  • Kruppel-Like Transcription Factors
  • Proto-Oncogene Proteins*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription Factors/physiology*
  • Zebrafish
  • Zebrafish Proteins*
  • Zinc Fingers/genetics
  • Zinc Fingers/physiology*
PubMed: 11553329 Full text @ Curr. Biol.
Hematopoietic cells arise from ventral mesoderm in different vertebrates, but the mechanisms through which various factors contribute to the hematopoietic processes, including erythrogenesis, remain incompletely understood. The Kruppel-like transcription factor Biklf is preferentially expressed in blood islands throughout zebrafish embryogenesis, marking the region of future erythropoiesis [1]. In this paper, we show that expression of biklf is significantly suppressed in the blood-less mutants vampire and m683 in which primitive hematopoiesis is impaired. Knockdown of biklf using morpholino-based antisense oligonucleotides (biklf-MO) led to a potent reduction in the number of circulating blood cells and deficiency in hemoglobin production. Consistently, we found that the expression of beta(e3)globin is strongly suppressed in biklf-MO-injected embryos, while gata1 expression is partly inhibited at the 10-somite stage. In addition, analysis of reporter constructs driven by the GATA1 and beta-globin promoters showed that Biklf can positively regulate both genes. These results indicate that Biklf is required for erythroid cell differentiation in zebrafish.