ZFIN ID: ZDB-PUB-010706-5
Cloning and characterization of zfBLP1, a Bcl-XL homologue from the zebrafish, Danio rerio
Chen, M., Gong, H., Cheng, C., Wang, J., Hong, J., and Wu, J.
Date: 2001
Source: BBA Gene Structure and Expression   1519(1-2): 127-133 (Journal)
Registered Authors: Cheng, Ching-Yi, Chen, Ming-chyuan, Gong, Hong-Yi, Hong, Jiann-Ruey, Wang, Jia-Pey, Wu, Jen-Leih
Keywords: apoptosis; Bcl-2; Bcl-XL; embryogenesis; zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Apoptosis
  • Base Sequence
  • Cloning, Molecular
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental*
  • Molecular Sequence Data
  • Proto-Oncogene Proteins c-bcl-2/biosynthesis
  • Proto-Oncogene Proteins c-bcl-2/genetics*
  • RNA, Messenger/biosynthesis
  • RNA, Messenger/isolation & purification
  • Sequence Alignment
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins
  • bcl-X Protein
PubMed: 11406282 Full text @ BBA Gene Structure and Expression
ABSTRACT
The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. We report here the cloning and characterization of a novel zebrafish Bcl-2 family protein, zfBLP1. The zfBLP1 cDNA is 1942 nucleotides long, encoding a polypeptide of 238 amino acids. The primary sequence of zfBLP1 shares 50% identity to human Bcl-XL, and contains all four conserved BH domains of the Bcl-2 family proteins. Primary sequence analysis identified a consensus ER retention signal at the C-terminal end of zfBLP1. Northern blot analysis indicated that there were two major and two minor zfBLP1 mRNA species expressed during embryonic development. Among the two major mRNA species, the short one, approx. 3 kb in size, was expressed throughout embryonic development, while the long one, approx. 7 kb long, was not detectable until the gastrula stage. These results suggest that zfBLP1 is a novel Bcl-2 family protein under complicated regulations, and is likely to play an important role in zebrafish oogenesis and embryogenesis.
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