PUBLICATION
Functional characterization and genetic mapping of alk8
- Authors
- Payne, T.L., Postlethwait, J.H., and Yelick, P.C.
- ID
- ZDB-PUB-010219-14
- Date
- 2001
- Source
- Mechanisms of Development 100(2): 275-289 (Journal)
- Registered Authors
- Payne-Ferreira, Tracie, Postlethwait, John H., Yelick, Pamela C.
- Keywords
- zebrafish; transforming growth factorb family; member signaling; activin-like-kinase receptors; bone morphogenetic protein signaling; dorsoventral patterning
- MeSH Terms
-
- Transcription, Genetic
- Humans
- Glycoproteins/biosynthesis
- In Situ Hybridization
- Mesoderm/metabolism
- Phylogeny
- Down-Regulation
- Tissue Distribution
- RNA, Messenger/metabolism
- Gene Expression Regulation, Developmental
- Chromosome Mapping
- Xenopus Proteins
- Protein Serine-Threonine Kinases/biosynthesis
- Protein Serine-Threonine Kinases/genetics*
- Bone Morphogenetic Protein 2
- Neurons/metabolism
- Conserved Sequence
- Zebrafish
- Zebrafish Proteins*
- Bone Morphogenetic Proteins/biosynthesis
- Models, Genetic
- Genes, Dominant
- Embryo, Nonmammalian/metabolism
- Up-Regulation
- Protein Biosynthesis
- Bone Morphogenetic Protein 4
- Phenotype
- Intercellular Signaling Peptides and Proteins*
- Polymorphism, Single-Stranded Conformational
- Animals
- Protein Structure, Tertiary
- Activin Receptors
- PubMed
- 11165484 Full text @ Mech. Dev.
Citation
Payne, T.L., Postlethwait, J.H., and Yelick, P.C. (2001) Functional characterization and genetic mapping of alk8. Mechanisms of Development. 100(2):275-289.
Abstract
The novel type I TGFbeta family member receptor alk8 is expressed both maternally and zygotically. Functional characterization of alk8 was performed using microinjection studies of constitutively active (CA), kinase modified/dominant negative (DN), and truncated alk8 mRNAs. CA Alk8 expression produces ventralized embryos while DN Alk8 expression results in dorsalized phenotypes. Truncated alk8 expressing embryos display a subtle dorsalized phenotype closely resembling that of the identified zebrafish dorsalized mutant, lost-a-fin (laf). Single-strand conformation polymorphism (SSCP) analysis was used to map alk8 to zebrafish LG02 in a region demonstrating significant conserved synteny to Hsa2, and which contains the human alk2 gene, ACVRI. Altogether, these functional, gene mapping and phylogenetic analyses suggest that alk8 may be the zebrafish orthologue to human ACVRI (alk2), and therefore extend previous studies of Alk2 conducted in Xenopus.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping