ZFIN ID: ZDB-PUB-010219-14
Functional characterization and genetic mapping of alk8
Payne, T.L., Postlethwait, J.H., and Yelick, P.C.
Date: 2001
Source: Mechanisms of Development   100(2): 275-289 (Journal)
Registered Authors: Payne-Ferreira, Tracie, Postlethwait, John H., Yelick, Pamela C.
Keywords: zebrafish; transforming growth factorb family; member signaling; activin-like-kinase receptors; bone morphogenetic protein signaling; dorsoventral patterning
MeSH Terms:
  • Activin Receptors
  • Animals
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Protein 4
  • Bone Morphogenetic Proteins/biosynthesis
  • Chromosome Mapping
  • Conserved Sequence
  • Down-Regulation
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental
  • Genes, Dominant
  • Glycoproteins/biosynthesis
  • Humans
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins*
  • Mesoderm/metabolism
  • Models, Genetic
  • Neurons/metabolism
  • Phenotype
  • Phylogeny
  • Polymorphism, Single-Stranded Conformational
  • Protein Biosynthesis
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases/biosynthesis
  • Protein-Serine-Threonine Kinases/genetics*
  • RNA, Messenger/metabolism
  • Tissue Distribution
  • Transcription, Genetic
  • Up-Regulation
  • Xenopus Proteins
  • Zebrafish
  • Zebrafish Proteins*
PubMed: 11165484 Full text @ Mech. Dev.
The novel type I TGFbeta family member receptor alk8 is expressed both maternally and zygotically. Functional characterization of alk8 was performed using microinjection studies of constitutively active (CA), kinase modified/dominant negative (DN), and truncated alk8 mRNAs. CA Alk8 expression produces ventralized embryos while DN Alk8 expression results in dorsalized phenotypes. Truncated alk8 expressing embryos display a subtle dorsalized phenotype closely resembling that of the identified zebrafish dorsalized mutant, lost-a-fin (laf). Single-strand conformation polymorphism (SSCP) analysis was used to map alk8 to zebrafish LG02 in a region demonstrating significant conserved synteny to Hsa2, and which contains the human alk2 gene, ACVRI. Altogether, these functional, gene mapping and phylogenetic analyses suggest that alk8 may be the zebrafish orthologue to human ACVRI (alk2), and therefore extend previous studies of Alk2 conducted in Xenopus.