|ZFIN ID: ZDB-PUB-001221-5|
Cloning and characterization of a novel nuclear Bcl-2 family protein, zfMcl-1a, in zebrafish embryo
Chen, M.C., Gong, H.Y., Cheng, C.Y., Wang, J.P., Hong, J.R., and Wu, J.L.
|Source:||Biochemical and Biophysical Research Communications 279(2): 725-731 (Journal)|
|Registered Authors:||Cheng, Ching-Yi, Chen, Ming-chyuan, Gong, Hong-Yi, Hong, Jiann-Ruey, Wang, Jia-Pey, Wu, Jen-Leih|
|Keywords:||apoptosis; Bcl-2 family proteins; nuclear Mcl-1; zebrafish; embryogenesis|
|PubMed:||11118352 Full text @ Biochem. Biophys. Res. Commun.|
Chen, M.C., Gong, H.Y., Cheng, C.Y., Wang, J.P., Hong, J.R., and Wu, J.L. (2000) Cloning and characterization of a novel nuclear Bcl-2 family protein, zfMcl-1a, in zebrafish embryo. Biochemical and Biophysical Research Communications. 279(2):725-731.
ABSTRACTThe importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. To elucidate the embryonic roles of Bcl-2 family proteins, we cloned and characterized the first zebrafish Bcl-2 family protein, zfMcl-1a. Zebrafish Mcl-1a shows the highest homology to rat Mcl-1 and contains several conserved BH domains of the Bcl-2 family proteins. It also contains a nuclear localization signal (NLS). Using EGFP reporter analysis, we verified the nuclear localization of zfMcl-1a. Deletion of the NLS resulted in distribution of the fusion protein in the cytoplasm. Northern blot analysis indicated that zfMcl-1a mRNA is 1.5 kb and was expressed in oocytes and throughout embryonic development. Notably, the expression of zfMcl-1a transcript was significantly downregulated during gastrulation. These results suggest that zfMcl-1a is a novel nuclear Bcl-2 family protein and is likely to play an important role in zebrafish oogenesis and embryogenesis.
- Genes / Markers (1)