ZFIN ID: ZDB-PUB-001221-5
Cloning and characterization of a novel nuclear Bcl-2 family protein, zfMcl-1a, in zebrafish embryo
Chen, M.C., Gong, H.Y., Cheng, C.Y., Wang, J.P., Hong, J.R., and Wu, J.L.
Date: 2000
Source: Biochemical and Biophysical Research Communications   279(2): 725-731 (Journal)
Registered Authors: Cheng, Ching-Yi, Chen, Ming-chyuan, Gong, Hong-Yi, Hong, Jiann-Ruey, Wang, Jia-Pey, Wu, Jen-Leih
Keywords: apoptosis; Bcl-2 family proteins; nuclear Mcl-1; zebrafish; embryogenesis
MeSH Terms:
  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Embryo, Nonmammalian/physiology*
  • Genes, Reporter
  • Green Fluorescent Proteins
  • Luminescent Proteins/genetics
  • Mice
  • Molecular Sequence Data
  • Myeloid Cell Leukemia Sequence 1 Protein
  • Neoplasm Proteins/chemistry
  • Neoplasm Proteins/genetics*
  • Neoplasm Proteins/isolation & purification
  • Protein Structure, Secondary
  • Proto-Oncogene Proteins c-bcl-2/chemistry
  • Proto-Oncogene Proteins c-bcl-2/genetics*
  • Proto-Oncogene Proteins c-bcl-2/isolation & purification
  • Rats
  • Recombinant Proteins/chemistry
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transfection
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins
PubMed: 11118352 Full text @ Biochem. Biophys. Res. Commun.
The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. To elucidate the embryonic roles of Bcl-2 family proteins, we cloned and characterized the first zebrafish Bcl-2 family protein, zfMcl-1a. Zebrafish Mcl-1a shows the highest homology to rat Mcl-1 and contains several conserved BH domains of the Bcl-2 family proteins. It also contains a nuclear localization signal (NLS). Using EGFP reporter analysis, we verified the nuclear localization of zfMcl-1a. Deletion of the NLS resulted in distribution of the fusion protein in the cytoplasm. Northern blot analysis indicated that zfMcl-1a mRNA is 1.5 kb and was expressed in oocytes and throughout embryonic development. Notably, the expression of zfMcl-1a transcript was significantly downregulated during gastrulation. These results suggest that zfMcl-1a is a novel nuclear Bcl-2 family protein and is likely to play an important role in zebrafish oogenesis and embryogenesis.