ZFIN ID: ZDB-PUB-001213-5
Functional interaction of vega2 and goosecoid homeobox genes in zebrafish
Kawahara, A., Wilm, T., Solnica-Krezel, L., and Dawid, I.B.
Date: 2000
Source: Genesis (New York, N.Y. : 2000)   28(2): 58-67 (Journal)
Registered Authors: Dawid, Igor B., Kawahara, Atsuo, Solnica-Krezel, Lilianna, Wilm, Thomas
Keywords: vega; bozozok/dharma; goosecoid; organizer formation
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Body Patterning/physiology*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/physiology
  • Gene Expression Regulation, Developmental
  • Goosecoid Protein
  • Homeodomain Proteins/chemistry
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism*
  • Luciferases/metabolism
  • Molecular Sequence Data
  • Mutagenesis
  • Organizers, Embryonic/embryology
  • Organizers, Embryonic/physiology*
  • Promoter Regions, Genetic
  • Protein Biosynthesis
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism*
  • Sequence Homology, Amino Acid
  • Transcription Factors*
  • Transfection
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 11064422 Full text @ Genesis
Summary: The gastrula organizer forms in the dorsal region of the zebrafish embryo, where the bozozok/dharma homeobox gene downregulates expression of the vega1 transcriptional repressor. Here, we describe a novel Vega family homeobox gene, vega2. Expression of vega2 is initiated at the ventral blastoderm margin during blastula stages, and by gastrulation becomes complementary to but partially overlapping with the dorsal expression domain of the homeobox gene goosecoid (gsc). This dorsal exclusion of vega2 expression is not observed in bozozok mutants in which organizer formation is impaired. Both vega2 and vega1 can physically interact with Gsc. Zebrafish embryos injected with vega2 mRNA failed to express gsc and developed a headless phenotype. Conversely, a putative dominant negative form of vega2, VP16-vega2, elicited the expansion of gsc expression and a dorsalized phenotype. We suggest that vega2, in cooperation with vega1, functions as a negative regulator of organizer genes including gsc, and participates in the refinement of the gastrula organizer domain. genesis 28:58-67, 2000.