PUBLICATION
Light acts directly on organs and cells in culture to set the vertebrate circadian clock
- Authors
- Whitmore, D., Foulkes, N.S., and Sassone-Corsi, P.
- ID
- ZDB-PUB-000824-4
- Date
- 2000
- Source
- Nature 404(6773): 87-91 (Journal)
- Registered Authors
- Foulkes, Nicholas-Simon, Sassone-Corsi, Paolo, Whitmore, David
- Keywords
- none
- MeSH Terms
-
- Zebrafish
- Light*
- Organ Culture Techniques
- Trans-Activators/genetics
- CLOCK Proteins
- Biological Clocks*/genetics
- Biological Clocks*/radiation effects
- Temperature
- Cell Line
- Heart/physiology
- Heart/radiation effects
- Circadian Rhythm*/genetics
- Circadian Rhythm*/radiation effects
- Kidney/physiology
- Kidney/radiation effects
- Animals
- PubMed
- 10716448 Full text @ Nature
Citation
Whitmore, D., Foulkes, N.S., and Sassone-Corsi, P. (2000) Light acts directly on organs and cells in culture to set the vertebrate circadian clock. Nature. 404(6773):87-91.
Abstract
The expression of clock genes in vertebrates is widespread and not restricted to classical clock structures. The expression of the Clock gene in zebrafish shows a strong circadian oscillation in many tissues in vivo and in culture, showing that endogenous oscillators exist in peripheral organs. A defining feature of circadian clocks is that they can be set or entrained to local time, usually by the environmental light-dark cycle. An important question is whether peripheral oscillators are entrained to local time by signals from central pacemakers such as the eyes or are themselves directly light-responsive. Here we show that the peripheral organ clocks of zebrafish are set by light-dark cycles in culture. We also show that a zebrafish-derived cell line contains a circadian oscillator, which is also directly light entrained.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping