ZFIN ID: ZDB-PUB-000810-5
Characterization of ZFOR1, a putative delta-opioid receptor from the teleost zebrafish (Danio rerio)
Rodriguez, R.E., Barrallo, A., Garcia Malvar, F., McFadyen, I.J., González Sarmiento, R., and Traynor, J.R.
Date: 2000
Source: Neuroscience letters   288(3): 207-210 (Journal)
Registered Authors: Barrallo Gimeno, Alejandro, González Sarmiento, Rogelio, Rodriguez, Raquel E.
Keywords: delta-opioid receptor; zebrafish; opioid binding; [S-35]GTPgamma S assays; HEK cells
MeSH Terms:
  • Animals
  • Binding Sites/drug effects
  • Diprenorphine/pharmacology
  • Humans
  • Narcotic Antagonists/pharmacology
  • Receptors, Opioid, delta/biosynthesis
  • Receptors, Opioid, delta/chemistry*
  • Receptors, Opioid, delta/metabolism
  • Sequence Homology, Amino Acid
  • Tritium
  • Zebrafish/metabolism*
  • Zebrafish/physiology*
PubMed: 10889344 Full text @ Neurosci. Lett.
ABSTRACT
ZFOR1 is a putative opioid receptor from zebrafish brain which has 66% homology with the mammalian delta-opioid receptor. When expressed in HEK293 cells ZFOR1 bound the non-selective opioid antagonist [(3)H]diprenorphine with high affinity. However, the binding of this ligand was not readily displaced by opioids selective for μ, delta or kappa opioid receptors (affinities>1000 nM). Rather non-selective ligands showed good affinity, as did the non-peptide delta-ligand BW373U86 (Ki 69 nM), the delta-antagonist naltrindole (Ki 28 nM) and the peptide beta-endorphin (Ki 37 nM). Agonist binding to the receptor encoded by ZFOR1 receptor stimulated the binding of [(35)S]GTPgammaS confirming coupling to G proteins. Study of the receptor should contribute to understanding of the evolution of the opioid system.
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