PUBLICATION
Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway
- Authors
- Dorsky, R.I., Raible, D.W., and Moon, R.T.
- ID
- ZDB-PUB-000201-22
- Date
- 2000
- Source
- Genes & Development 14(2): 158-162 (Journal)
- Registered Authors
- Dorsky, Richard, Moon, Randall T., Raible, David
- Keywords
- Wnt; beta-catenin; nacre; neural crest; MITF
- MeSH Terms
-
- Animals
- Cell Division/genetics
- DNA-Binding Proteins/genetics*
- Gene Expression Regulation, Developmental*
- Melanophores/physiology*
- Microphthalmia-Associated Transcription Factor
- Molecular Sequence Data
- Pigmentation/genetics*
- Protein-Tyrosine Kinases/metabolism
- Protein-Tyrosine Kinases/physiology
- Proto-Oncogene Proteins/physiology*
- Sequence Homology, Nucleic Acid
- Signal Transduction/genetics
- Transcription Factors*
- Wnt Proteins
- Zebrafish/genetics*
- Zebrafish Proteins*
- PubMed
- 10652270 Full text @ Genes & Dev.
Citation
Dorsky, R.I., Raible, D.W., and Moon, R.T. (2000) Direct regulation of nacre, a zebrafish MITF homolog required for pigment cell formation, by the Wnt pathway. Genes & Development. 14(2):158-162.
Abstract
We have shown that Wnt signals are necessary and sufficient for neural crest cells to adopt pigment cell fates. nacre, a zebrafish homolog of MITF, is required for pigment cell differentiation. We isolated a promoter region of nacre that contains Tcf/Lef binding sites, which can mediate Wnt responsiveness. This promoter binds to zebrafish Lef1 protein in vitro, and a nacre reporter construct is strongly repressed by dominant-negative Tcf in melanoma cells. Mutation of Tcf/Lef sites abolishes Lef1 binding and reporter function in vivo. Wnt signaling therefore directly activates nacre, which in turn leads to pigment cell differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping