ZFIN ID: ZDB-PUB-000125-10
Temporal expression and T3 induction of thyroid hormone receptors alpha1 and beta1 during early embryonic and larval development in zebrafish, Danio rerio
Liu, Y.-W., Lo, L-J., and Chan, W.-K.
Date: 2000
Source: Molecular and Cellular Endocrinology   159(1-2): 187-195 (Journal)
Registered Authors: Chan, Woon-Khiong, Liu, Yi-wen
Keywords: thyroid hormone receptor; zebrafish; embryonic development; larvae
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers/genetics
  • DNA, Complementary/genetics
  • Female
  • Gene Expression/drug effects
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Receptors, Thyroid Hormone/biosynthesis*
  • Receptors, Thyroid Hormone/genetics*
  • Tissue Distribution
  • Transcriptional Activation
  • Transfection
  • Triiodothyronine/pharmacology*
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
PubMed: 10687864 Full text @ Mol. Cell. Endocrinol.
ABSTRACT
The effects of thyroid hormones on metabolism and development are mediated by thyroid hormone receptors (TRs). We report the cloning and characterization of a TR1 cDNA from zebrafish. Southern blot analysis revealed that there is a single genomic locus for the TR gene, while the TR gene potentially has two loci. Multiple TR and TR transcripts were detected in adult tissues. Using a semiquantitative RT-PCR assay, zygotic expression of TR1 and TR1 were shown to occur before the midblastula transition stage. In transiently transfected HeLa cells, TR1 displayed constitutive transactivation in the absence of ligands, which was slightly enhanced by triiodothyronine (T3). The transactivating activity of TR1 was strictly ligand-dependent and repressed in the absence of T3. Finally, the T3 induction of TR1 and TR1 mRNAs was demonstrated in zebrafish embryos and larvae. The auto-induction of TR1 and TR1 may serve a regulatory role during the embryonic and larval development of zebrafish.
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