ZFIN ID: ZDB-LAB-210827-1
Anbalagan Lab
PI/Director: Anbalagan, Savani
Contact Person: Anbalagan, Savani
Email: savanb@amu.edu.pl
URL: http://ibmib.amu.edu.pl/en/main-page/
Address: Institute of Molecular Biology & Biotechnology, Faculty of Biology, Adam Mickiewicz University, ul. Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
Country: Poland
Phone: +48618295905
Line Designation: amu


Oxytocin (OXT) is a hypothalamus-derived neuropeptide majorly secreted via the posterior pituitary. In OXT neurons, dendritic and axonal synapses store and release OXT during social and reproductive behavior. Reduced hypothalamic OXT content and genetic polymorphisms in oxytocin receptor are observed in autism-like mice models and socially-impaired children respectively. These observations led to the controversial OXT-deficit hypothesis of autism spectrum disorders. Thus, investigating the molecular mechanisms that regulate synaptic OXT content and release are essential to understand autism spectrum disorders.

At a molecular level, glial pituicytes that are closely-associated with the neurohypophyseal axonal terminals can secrete factors that affect OXT content or release. However, the identity and the role of pituicyte-derived secreted factors that can regulate synaptic OXT are largely unknown. Finally, situated outside the blood-brain barrier (BBB), glial pituicytes are directly exposed to the blood-borne molecules and are highly responsive to physiological challenges to promote synaptic plasticity. But the identity and the role of the pituicyte-derived cues that regulate the plasticity of OXT axonal terminals and OXT neuropeptide content upon challenges are largely unknown.

Our lab combines molecular and advanced light microscopy techniques to study the role of glial pituicytes.

Funding information:
National Science Centre Sonata-BIS 2020/38/E/NZ3/00090

Wysocka, Emilia Technical Staff

Ribeiro, D., Nunes, A.R., Teles, M., Anbalagan, S., Blechman, J., Levkowitz, G., Oliveira, R.F. (2020) Genetic variation in the social environment affects behavioral phenotypes of oxytocin receptor mutants in zebrafish. eLIFE. 9:
Nunes, A.R., Carreira, L., Anbalagan, S., Blechman, J., Levkowitz, G., Oliveira, R.F. (2020) Perceptual mechanisms of social affiliation in zebrafish. Scientific Reports. 10:3642
Ribeiro, D., Nunes, A.R., Gligsberg, M., Anbalagan, S., Levkowitz, G., Oliveira, R.F. (2020) Oxytocin receptor signaling modulates novelty recognition but not social preference in zebrafish. Journal of neuroendocrinology. 32(4):e12834
Anbalagan, S., Blechman, J., Gliksberg, M., Gordon, L., Rotkopf, R., Dadosh, T., Shimoni, E., Levkowitz, G. (2019) Robo2 regulates synaptic oxytocin content by affecting actin dynamics. eLIFE. 8:
Anbalagan, S., Gordon, L., Blechman, J., Matsuoka, R.L., Rajamannar, P., Wircer, E., Biran, J., Reuveny, A., Leshkowitz, D., Stainier, D.Y.R., Levkowitz, G. (2018) Pituicyte Cues Regulate the Development of Permeable Neuro-Vascular Interfaces. Developmental Cell. 47(6):711-726.e5
Blechman, J., Anbalagan, S., Matthews, G.G., Levkowitz, G. (2018) Genome Editing Reveals Idiosyncrasy of CNGA2 Ion Channel-Directed Antibody Immunoreactivity Toward Oxytocin. Frontiers in cell and developmental biology. 6:117