ZFIN ID: ZDB-LAB-180227-1
Tim Czopka Lab
PI/Director: Czopka, Tim
Contact Person: Czopka, Tim
Email: Tim.Czopka@ed.ac.uk
URL: https://www.czopka-lab.com
Address: - University of Edinburgh Centre for Clinical Brain Sciences Chancellor's Building 49 Little France Crescent Edinburgh EH16 4SB AND Institute of Neuronal Cell Biology Technical University of Munich Biedersteiner Str. 29 80802 Munich Germany -
Country: United Kingdom
Phone: +44 131 242 6395
Line Designation: tum

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My lab aims to understand how oligodendrocytes communicate with neurons, and how these interactions affect the brain.

About 5% of all brain cells are undifferentiated oligodendrocyte precursor cells which tile the brain throughout life. These cells sense nervous system activity and represent the cellular source for new myelin during long-term development, plastic adaptations, and CNS regeneration. However, there are many more oligodendrocyte precursors than ever differentiate, but which still constantly communicate with surrounding neurons and other CNS cells. How this cell population can be triggered to produce new myelin, and how the non-myelinating oligoendrocytes affect nervous system function, remains unclear.

To address this, we use zebrafish as model organism and a wide range of complementary methods including high-resolution optical microscopy of live cell reporters, optophysiology and biomolecular sensor imaging, cellular genetic manipulations, and behavioural analysis.

Xiao, Yan Post-Doc Marsica, Roberta Graduate Student Vagionitis, Stavros Graduate Student
Hoodless, Laura Research Staff

Siems, S.B., Jahn, O., Hoodless, L.J., Jung, R.B., Hesse, D., Möbius, W., Czopka, T., Werner, H.B. (2021) Proteome Profile of Myelin in the Zebrafish Brain. Frontiers in cell and developmental biology. 9:640169
Marisca, R., Hoche, T., Agirre, E., Hoodless, L.J., Barkey, W., Auer, F., Castelo-Branco, G., Czopka, T. (2020) Functionally distinct subgroups of oligodendrocyte precursor cells integrate neural activity and execute myelin formation. Nature Neuroscience. 23(3):363-374
Tian, W., Czopka, T., López-Schier, H. (2020) Systemic loss of Sarm1 protects Schwann cells from chemotoxicity by delaying axon degeneration. Communications biology. 3:49
Lozano-Ortega, M., Valera, G., Xiao, Y., Faucherre, A., López-Schier, H. (2018) Hair cell identity establishes labeled lines of directional mechanosensation. PLoS Biology. 16:e2004404
Almeida, R.G., Pan, S., Cole, K.L.H., Williamson, J.M., Early, J.J., Czopka, T., Klingseisen, A., Chan, J.R., Lyons, D.A. (2018) Myelination of Neuronal Cell Bodies when Myelin Supply Exceeds Axonal Demand. Current biology : CB. 28(8):1296-1305.e5
Auer, F., Vagionitis, S., Czopka, T. (2018) Evidence for Myelin Sheath Remodeling in the CNS Revealed by In Vivo Imaging. Current biology : CB. 28(4):549-559.e3
Vagionitis, S., Czopka, T. (2018) Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1791:25-35
Karttunen, M.J., Czopka, T., Goedhart, M., Early, J.J., Lyons, D.A. (2017) Regeneration of myelin sheaths of normal length and thickness in the zebrafish CNS correlates with growth of axons in caliber. PLoS One. 12:e0178058
Hoodless, L.J., Lucas, C.D., Duffin, R., Denvir, M.A., Haslett, C., Tucker, C.S., Rossi, A.G. (2016) Genetic and pharmacological inhibition of CDK9 drives neutrophil apoptosis to resolve inflammation in zebrafish in vivo. Scientific Reports. 5:36980
Czopka, T. (2016) Insights into mechanisms of central nervous system myelination using zebrafish. Glia. 64(3):333-49
Hoodless, L.J., Robb, C.T., Felton, J.M., Tucker, C.S., Rossi, A.G. (2016) Models for the Study of the Cross Talk Between Inflammation and Cell Cycle. Methods in molecular biology (Clifton, N.J.). 1336:179-209
Xiao, Y., López-Schier, H. (2016) Studying Axonal Regeneration by Laser Microsurgery and High-Resolution Videomicroscopy. Methods in molecular biology (Clifton, N.J.). 1451:271-80
Nawaz, S., Sánchez, P., Schmitt, S., Snaidero, N., Mitkovski, M., Velte, C., Brückner, B.R., Alexopoulos, I., Czopka, T., Jung, S.Y., Rhee, J.S., Janshoff, A., Witke, W., Schaap, I.A., Lyons, D.A., Simons, M. (2015) Actin filament turnover drives leading edge growth during myelin sheath formation in the central nervous system. Developmental Cell. 34:139-51
Mensch, S., Baraban, M., Almeida, R., Czopka, T., Ausborn, J., El Manira, A., Lyons, D. A. (2015) Synaptic vesicle release regulates myelin sheath number of individual oligodendrocytes in vivo. Nature Neuroscience. 18:628-630
Lucas, C.D., Hoodless, L.J., Rossi, A.G. (2014) Swimming against the tide: drugs drive neutrophil reverse migration. Science Translational Medicine. 6:225fs9
Czopka, T., Ffrench-Constant, C., and Lyons, D.A. (2013) Individual Oligodendrocytes Have Only a Few Hours in which to Generate New Myelin Sheaths In Vivo. Developmental Cell. 25(6):599-609
Lucas, C.D., Allen, K.C., Dorward, D.A., Hoodless, L.J., Melrose, L.A., Marwick, J.A., Tucker, C.S., Haslett, C., Duffin, R., and Rossi, A.G. (2013) Flavones induce neutrophil apoptosis by down-regulation of Mcl-1 via a proteasomal-dependent pathway. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 27(3):1084-1094
Almeida, R.G., Czopka, T., Ffrench-Constant, C., and Lyons, D.A. (2011) Individual axons regulate the myelinating potential of single oligodendrocytes in vivo. Development (Cambridge, England). 138(20):4443-50
Czopka, T., and Lyons, D.A. (2011) Dissecting mechanisms of myelinated axon formation using zebrafish. Methods in cell biology. 105:25-62