ZFIN ID: ZDB-LAB-120515-2
Pantazis Lab
PI/Director: Pantazis, Periklis (Laki)
Contact Person: Pantazis, Periklis (Laki)
Email: p.pantazis@imperial.ac.uk
URL: http://www.pantazis-lab.org
Address: Imperial College London Department of Bioengineering South Kensington Campus London SW7 2AZ
Country: United Kingdom
Phone: +44 (0)20 7594 6367
Line Designation: zh

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The Laboratory of NanoBioImaging combines cutting-edge imaging technologies with sophisticated quantitative imaging analyses to investigate early development and tissue regeneration in vivo. For this purpose, we apply confocal and non-linear imaging modalities, including two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging.

We invented SHG nanoprobes as superior imaging probes, enabling high contrast time-lapse imaging deep within tissues without any signal decay over time. Since their optical signal is based on scattering, rather than absorption, they neither bleach nor blink, and their signal does not saturate. They can be detected with little background signal and are physiologically inert. We are currently pioneering uses of these nanolabels for clinically relevant applications.
In another line of research, we developed the so-called PhOTO zebrafish. PhOTO zebrafish combine the benefits of global and sparse embryo labeling for cell tracking in the early embryo and extends this ability to monitor the contribution of labeled cells during adult stage epimorphic regeneration.

Finally, we established a quantitative imaging assay, the Fluoresence Decay After Photoactivation (FDAP) assay, as an optimal tool for dissecting early pluripotency decisions in developing mammalian embryos.


Sonay, A.Y., Kalyviotis, K., Yaganoglu, S., Unsal, A., Konantz, M., Teulon, C., Lieberwirth, I., Sieber, S., Jiang, S., Behzadi, S., Crespy, D., Landfester, K., Roke, S., Lengerke, C., Pantazis, P. (2021) Biodegradable Harmonophores for Targeted High-Resolution In Vivo Tumor Imaging. ACS nano. 15(3):4144-4154
Malkinson, G., Mahou, P., Chaudan, É., Gacoin, T., Sonay, A.Y., Pantazis, P., Beaurepaire, E., Supatto, W. (2020) Fast In Vivo Imaging of SHG Nanoprobes with Multiphoton Light-Sheet Microscopy. ACS photonics. 7:1036-1049
Mohr, M.A., Argast, P., Pantazis, P. (2016) Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins. Nature Protocols. 11:2419-2431
Mohr, M.A., Pantazis, P. (2016) Single neuron morphology in vivo with confined primed conversion. Methods in cell biology. 133:125-138
Dempsey, W.P., Hodas, N.O., Ponti, A., Pantazis, P. (2015) Determination of the source of SHG verniers in zebrafish skeletal muscle. Scientific Reports. 5:18119
Dempsey, W.P., Georgieva, L., Helbling, P.M., Sonay, A.Y., Truong, T.V., Haffner, M., Pantazis, P. (2015) In vivo single-cell labeling by confined primed conversion. Nature Methods. 12(7):645-8
Pantazis, P., Supatto, W. (2014) Advances in whole-embryo imaging: a quantitative transition is underway. Nature reviews. Molecular cell biology. 15:327-39
Dempsey, W.P., Qin, H., Pantazis, P. (2014) In Vivo Cell Tracking Using PhOTO Zebrafish. Methods in molecular biology (Clifton, N.J.). 1148:217-28
Mikut, R., Dickmeis, T., Driever, W., Geurts, P., Hamprecht, F.A., Kausler, B.X., Ledesma-Carbayo, M.J., Marée, R., Mikula, K., Pantazis, P., Ronneberger, O., Santos, A., Stotzka, R., Strähle, U., and Peyriéras, N. (2013) Automated Processing of Zebrafish Imaging Data: A Survey. Zebrafish. 10(3):401-21
Culi-Viskota, J., Dempsey, W.P., Fraser, S.E., and Pantazis, P. (2012) Surface functionalization of barium titanate SHG nanoprobes for in vivo imaging in zebrafish. Nature Protocols. 7(9):1618-1633
Dempsey, W.P., Fraser, S.E., and Pantazis, P. (2012) PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development and Regeneration. PLoS One. 7(3):e32888
Caneparo, L., Pantazis, P., Dempsey, W., and Fraser, S.E. (2011) Intercellular bridges in vertebrate gastrulation. PLoS One. 6(5):e20230
Pantazis, P., Maloney, J., Wu, D., and Fraser, S.E. (2010) Second harmonic generating (SHG) nanoprobes for in vivo imaging. Proceedings of the National Academy of Sciences of the United States of America. 107(33):14535-14540