Figure 1
- ID
- ZDB-IMAGE-260608-1
- Publication
- Li et al., 2026 - TRIM25 enhances hypoxia signaling by catalyzing K11-linked polyubiquitination and stabilization of HIF-α
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- Figures for Li et al., 2026
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Figure 1
TRIM25 enhances hypoxia signaling.A, immunoblotting (IB) of overexpressed Flag-TRIM25 in U251 cells. B–D, quantitative real-time PCR (qPCR) analysis of PDK1, VEGF, and GLUT1 mRNA in U251 cells transfected with Flag-empty vector or Flag-TRIM25 for 12 h, followed by treatment with or without CoCl2 (200 μM) for 12 h. E–H, qPCR analysis of PGK1, PDK1, VEGF, and GLUT1 mRNA in U251 cells transfected with Flag-empty vector or Flag-TRIM25 for 8 h under normoxia (21% O2), followed by normoxia (21% O2) or hypoxia (1% O2) treatment for 16 h. I, IB of TRIM25 expression in wild-type (TRIM25+/+) and TRIM25-deficient (TRIM25−/−) U251 cells. J–L, qPCR analysis of PDK1, VEGF, and GLUT1 mRNA in wild-type (TRIM25+/+) and TRIM25-deficient (TRIM25−/−) U251 cells treated with or without CoCl2 (200 μM) for 12 h. M–P, qPCR analysis of EPO, PDK1, VEGF, and GLUT1 mRNA in wild-type (TRIM25+/+) and TRIM25-deficient (TRIM25−/−) U251 cells treated with normoxia (21% O2) or hypoxia (1% O2) for 16 h. Data in (B–H, J–P) are shown as mean ± SD; statistical significance was determined by unpaired two-tailed Student’s t test; each point represents a technical replicate of a representative experiment from three independent experiments.