PUBLICATION

TRIM25 enhances hypoxia signaling by catalyzing K11-linked polyubiquitination and stabilization of HIF-α

Authors
Li, Z., Li, J., Li, Z., Wang, R., Yuan, L., Song, Y., Wang, Y., Yan, R., Lai, F., Wang, J., Xiao, W.
ID
ZDB-PUB-260509-8
Date
2026
Source
The Journal of biological chemistry : 113125113125 (Journal)
Registered Authors
Li, Zhi, Wang, Jing, Xiao, Wuhan
Keywords
HIF-α, Hypoxia signaling, TRIM25, Ubiquitination
MeSH Terms
none
PubMed
42103230 Full text @ J. Biol. Chem.
Abstract
TRIM25 is an E3 ubiquitin ligase involved in various cellular processes due to its enzymatic activity. In particular, it plays a role in antiviral innate immunity. Here, we demonstrate that TRIM25 modulates hypoxia signaling. TRIM25 interacts with HIF-1α and HIF-2α, stabilizing them. TRIM25 catalyzes K11-linked polyubiquitination of HIF-1α at K719 and K721 and of HIF-2α at K709. This results in the stabilization of the proteins and enhanced hypoxia signaling. Moreover, TRIM25-mediated augmentation of hypoxia signaling depends on HIF-1α. Trim25-deficient mice are more sensitive to hypoxia, and zebrafish lacking trim25 show a similar phenotype. These data reveal TRIM25's role in regulating hypoxia signaling and provide insight into a new mechanism that modulates the stabilization and activity of HIF-1α and HIF-2α.
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