Figure 4

Figure 4

SERPINA1 is expressed and secreted by podocytes. (A) SERPINA1 mRNA expression in cultured human podocytes. Expression is given as fold change over time compared with 0 hour. Expression levels are normalized to GAPDH. (B) SERPINA1 protein in supernatant of cultured human podocytes at 16 and 24 hours relative to 1 hour; n=3 independent experiments per group. (C) SERPINA1 protein in supernatant of cultured human podocytes at 1, 16, and 24 hours after treatment with DEPC water (control) or a with a PAR1 agonist; n=3 independent experiments per group. (D) SERPINA1 protein in supernatant of cultured human podocytes 24 hours after treatment with DEPC water (control) or with TGF-β1; n=8 independent experiments per group. All data shown as mean of fold change ±SD. **P < 0.01, ***P < 0.001. DEPC, diethyl pyrocarbonate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PAR1, protease-activated receptor 1.