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Fig. 1 Generation and molecular characterization of the keap1bdl40 knockout allele using CRISPR/Cas9. A Gene structure and deletion. Zebrafish keap1b gene showing gRNA target site (red arrow) at the 5’-UTR/exon 2 boundary. The 40-bp deletion removes 10 bp from 5’-UTR, the ATG start codon (3 bp), and 27 bp from exon 2. Translation initiates from a downstream out-of-frame ATG (green), producing a 20-amino-acid peptide (MGTASSATRWRATRPPPSPS*) terminated by a premature stop codon (asterisk). B Protein products. Wild-type: 593-aa protein with BTB, IVR, and DGR domains. Mutant: 20-aa non-functional peptide (orange) lacking all domains. C PCR genotyping. Gel electrophoresis showing wild-type (177 bp), mutant (137 bp), and heterozygous (both bands) genotypes