Figure 9

Figure 9 Kif1aa mutants have normal mechanosensitive and presynaptic responses

A, overview of the scheme used to assess evoked calcium responses in lateral‐line hair cells. A fluid jet is used to deliver flow stimuli to lateral‐line neuromasts. A membrane‐localized GCaMP6s (myo6b:memGCaMP6s, green) expressed in hair cells is used to measure fluid jet‐evoked calcium signals in apical hair bundles or presynaptic calcium signals at the cell base. B–E, top‐down images show optical planes of memGCaMP6s in neuromast hair bundles (B,D) or at the presynapse (C,E). Heatmaps show spatial representations of ∆ GCaMP signals during evoked mechanosensitive (B,C) and presynaptic (D,E) activity during a 500 ms stimulation (stim) compared with pre‐stimulus (prestim) in sibling controls and kif1aa mutants. F–I, traces show the average mechanosensitive (F) and calcium presynaptic (H) calcium responses in sibling control and kif1aa mutant hair cells (n = 8 neuromasts). Dot plots show that the average mechanosensitive (G) and presynaptic (I) calcium responses are similar in sibling control and kif1aa mutant hair cells (G, control: 86.1 ± 24.2, kif1aa: 78.9 ± 18.7, n = 8 control and kif1aa neuromasts, unpaired t test, P = 0.514; I, control: 121.7 ± 57.6, kif1aa: 124.1 ± 51.5, n = 8 control and kif1aa neuromasts, unpaired t test, P = 0.930, 5 dpf). Scale bars in B–E = 5 µm.