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Fig. 6 CITED4 is required for hiPSC-derived CM maturation. (A) Experimental timeline. (B) qPCR analysis for CITED4 expression in hiPSC-CMs following treatment with CITED4 siRNA or scramble control siRNA. (C-D′) ACTN2-eGFP hiPSC-CMs following treatment with scramble control (C,C′) or CITED4 (D,D′). Magnification of dashed boxed areas below show sarcomere structure based on ACTN2-GFP expression. (E,F) Quantification of sarcomere length in hiPSC-CMs at day 17 (E) and day 22 (F) following treatment with CITED4 siRNA or scramble control siRNA. (G) Gene expression ratios between TNNI3/TNNI1 and MYH7/MYH6 measured by qPCR in hiPSC-CMs following treatment with CITED4 siRNA or scramble control siRNA. Data are mean±s.e.m. Unpaired two-tailed t-test. Scale bars: 20 µm.