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Fig. 3 The mRNA sequencing analysis suggests bmp2a as a promising candidate as a key regulator of bone formation in zebrafish treated with Ginsenoside Re. A. Significant differences in mineralization metabolic pathways revealed by KEGG pathway enrichment analysis in zebrafish treated with Ginsenoside Re. B. Differential gene GO classification analysis indicates predominance of metabolic processes among differentially expressed genes in zebrafish treated with Ginsenoside Re. C. Differential gene clustering Heat Map reveals trpv6 and bmp2a genes as potential candidates for Ginsenoside Re-mediated osteogenesis promotion in zebrafish tail fin amputation model. D. Ginsenoside Re induces downregulation of trpv6 gene expression in the zebrafish tail fin regeneration model. E. Ginsenoside Re induces upregulation of bmp2a gene expression in the zebrafish tail fin regeneration model. Statistical methods: two-tailed Student's t-test, (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).