FIGURE 6

FIGURE 6

Zebrafish anxiety-like behavior and social interaction improved after vitamin A intervention. (A) Open field path map of zebrafish. (B) Total distance of zebrafish open field movement (n = 10). (C) Average speed of zebrafish open field (n = 10). (D) The proportion of distance between central and marginal areas of zebrafish (Edge area distance ratio = outer distance/total distance, central area distance ratio = inner distance/total distance) (n = 10). (E) Diagram of a zebrafish social interaction experiment. (F) Zebrafish contacts per min (Contact is defined as the distance between two fish ≤ one body length) (n = 12). (G) Zebrafish contact duration ratio (n = 12). (H) Zebrafish Minimum distance between two fish (n = 10). (I) Average distance between two zebrafish (n = 10). These data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus DMSO group.