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Fig. 4 - Supplemental 2 Examination on the involvement of apoptotic process in epithelial cells and expression of inflammation-related genes in neutrophils and B cells in the intestines of ctla-4-/- zebrafish. (A) Expression map of the epithelial markers within the cell populations of the zebrafish intestines. (B) Expression map of the neutrophil markers within the cell populations of the zebrafish intestines. (C) KEGG enrichment bar plot of all differentially expressed genes (DEGs) from epithelial cells. (D) Quantification of TUNEL-positive cells per 1×104 μm2 in WT and ctla-4-/- posterior intestines (n=5). (E) Heatmap of inflammation-related genes in B cells from wild-type and ctla-4-/- intestines. Statistical significance was assessed through an unpaired Student’s t-test (***p < 0.001).