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Fig. 3 Impact of Socs3a ablation on emergency granulopoiesis and neutrophil function. (A–E) Socs3a wildtype (WT) and knockout (KO) embryos on a Tg(mpx:GFP) background were injected with LPS (+LPS) at 3 dpf and imaged 8 hours later showing (A–D) representative images and (E) quantitation of total mpx+ cells. (F,G) Embryos were separately inflicted with a tailfin wound at 3 dpf, with the caudal fin imaged at 4 h time points from 0 to 24 hours post wounding, with representative images (G) and quantification of (F) the mpx+ cells present at the site of wounding shown. Scale bars of 100 µm are shown for (A–D,G), with the mean ± SEM and statistical significance shown for (E,F) (** p < 0.01; ns, not significant; n = 8–12).